[Novoprotein] Recombinant Human Cyclin-Dependent Kinase 4/CXK4 (N-6His)

Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Recombinant Human Cyclin-Dependent Kinase 4 is produced by our E.coli expression system & the target gene encoding Met1-Glu303 is expressed with a 6His, T7 tag at the N-terminus.

Cyclin-Dependent Kinase 4 (CDK4) is a member of the CMGC Ser/Thr protein kinase family & CDC2/CDKX subfamily. CDK4 is a component of Cyclin D-CDK4 (DC) complexes that phosphorylate & inhibit members of the retinoblastoma (RB) protein family including RB1 & regulate the cell-cycle during G1/S transition. These complexes are major integrators of various mitogenenic & antimitogenic signals. It is shown that CDK4 is responsible for the phosphorylation of retinoblastoma gene product (Rb). Defects in CDK4 are a cause of susceptibility to cutaneous Malignant Melanoma Type 3.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.