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Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer Protein, His,Avitag™ (MALS verified) Europäischer Partner

ArtNr SPN-H82E3-25ug
Hersteller ACROBiosystems
Menge 25 ug
Quantity options 200 ug 25 ug
Kategorie
Typ Proteins Recombinant
Format Solid
Specific against Coronavirus
Host HEK293
Konjugat/Tag Biotin, HIS, Avi
Purity 95%
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Spike,S protein,Spike glycoprotein,S glycoprotein
Versandbedingung Raumtemperatur
Lieferbar
Specificity HCoV-HKU1
Manufacturer - Category
Protein / MABSol® Biotin Labeled Proteins
Manufacturer - Conjugate / Tag
C-10xHis & C-Avi, Biotin-labeled
Shipping Temperature
RT
Storage Conditions
-20°C
Molecular Weight
147.2 kDa
Manufacturer - Format
Powder
Description
Biotinylated HCoV-HKU1 (isolate N5) Spike Trimer, His, Avitag (SPN-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ala 13 - Asn 1276 (Accession # Q0ZME7-1). Amino acid substitutions RRKRR 752-756 GGSGS and NL 1067-1068 PP are introduced to abolish the furin cleavage site and stabilize the trimeric prefusion state of the spike protein, respectively.
Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Molecule
Spike protein
Exp Region
Ala 13 - Asn 1276
Characteristics
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 147.2 kDa. The protein migrates as 200-220 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
1.0 EU per μg
Buffer
0.1 M Sodium citrate, pH 5.5
Stability
● -20°C to -70°C for 12 months in lyophilized state;
● -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Protectant
trehalose

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 ug
Lieferbar: In stock
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