TransStart® TopTaq DNA Polymerase

PCR Enzyme

TransStart® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.


• Compared with TransStart® Taq DNA Polymerase, TransStart®TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
• TransStart® TopTaq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• The specificity is higher than antibody based or chemically modified hot start DNA polymerases.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.


Applications
• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR


Storage
at -20 ℃ for two years


Shipping
Dry ice (-70 ℃)