Rabies Virus Nucleocapsid, Recombinant, Yeast (CVS-11)

Blue Ice

56

Supplied as a liquid in PBS, pH 7.4. No preservative added.

The rabies virus is a member of the Lyssavirus genus, which have helical symmetry, so their infectious particles are ~cylindrical in shape. They are characterized by an extremely broad host spectrum ranging from plants to insects and mammals; human- infecting viruses more commonly have cubic symmetry and take shapes approximating regular polyhedron. The virus has a bullet like shape with a length of about 180nm and a cross-sectional diameter of about 75nm. One end is rounded or conical and the other end is planar or concave. The lipoprotein envelope carries knob-like spikes composed of Glycoprotein G. Spikes do not cover the planar end of the virion (virus particle). Beneath the envelope is the membrane or matrix (M) protein layer which may be invaginated at the planar end. The core of the virion consists of helically arranged ribonucleoprotein. Rapid and accurate laboratory diagnosis of rabies in humans and other animals are essential for timely administration of post exposure prophylaxis. The nature of rabies disease dictates that laboratory tests be standardized, rapid, sensitive, specific, economical, and reliable. The standard test for rabies testing is dFA.

Rabies Virus nucleocapsid encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases.
Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds protein P and thereby positions the polymerase on the template. Protein P acts as a chaperone on free protein N to prevent it from aggregation before encapsidating genomic RNA.

Source:
Recombinant protein corresponding to 450aa from Rabies Virus Nuceloprotein, fused to his-tag, expressed in S. cereviasae.

Molecular Weight:
~56kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: coat at 1-2ug/ml
Western Blot: load 100-500ng protein/lane
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.