ATG3, Recombinant, Human, aa1-314, His-Tag (Autophagy-Related Protein 3, Ubiquitin Like-Conjugating Enzyme ATG3 Isoform 1, APG3, APG3-LIKE, APG3L, PC3-96)

Blue Ice

38, 3

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 10% glycerol.

Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy.

Source:
Recombinant protein corresponding to aa1-314 from human ATG3, fused to His-tag at N-terminus, expressed in E.coli.

Molecular Weight:
~38.3kD (337aa), confirmed by MALDI-TOF

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Applications:
Suitable for use in SDS-PAGE. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.