MANF, Recombinant, Human, aa25-182, His-Tag (Mesencephalic astrocyte-derived neurotrophic factor)

Blue Ice

20, 8

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 10% glycerol.

Mesencephalic astrocyte-derived neurotrophic factor, also known as MANF, is a 20kD member of the ARMET family of proteins. MANF was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

Source:
Recombinant protein corresponding to aa25-182 of human MANF, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~20.8kD

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMLRPGD CEVCISYLGR FYQDLKDRDV TFSPATIENE LIKFCREARG KENRLCYYIG ATDDAATKII NEVSKPLAHH IPVEKICEKL KKKDSQICEL KYDKQIDLST VDLKKLRVKE LKKILDDWGE TCKGCAEKSD YIRKINELMP KYAPKAASAR TDL

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.