TSC22D3, Recombinant, Human, aa1-134, His-Tag (TSC22 Domain family protein 3, DIP, DSIPI, GILZ, hDIP, TSC-22R)

Blue Ice

17, 2

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.

TSC22 domain family protein 3, also known as TSC22D3, protects T-cells from IL2 deprivation- induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, TSC22D3 plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, TSC22D3 inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities.

Recombinant protein corresponding to aa1-134 from human TSC22D3, fused to His-tag at N-terminal expressed in E. coli.

Molecular Weight:
~17.2kD(157aa)

Amino Acid Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMNTEMYQ TPMEVAVYQL HNFSISFFSS LLGGDVVSVK LDNSASGASV VAIDNKIEQA MDLVKNHLMY AVREEVEILK EQIRELVEKN SQLERENTLL KTLASPEQLE KFQSCLSPEE PAPESPQVPE APGGSAV

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.