Anti-TNF-alpha Antibody

Antibodies|Primary Antibodies

-20°C

Goat Anti-Human TNF-alpha

Phosphate buffered saline

Purified IgG - lyophilized

Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

This product is specific for human Tumour Necrosis Factor alpha (TNF❏❏alpha), a 17.4kDa multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-ÐB, the induction of apoptosis in various tumour cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Antisera to human TNF-alpha were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.

*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.