Human Angiogenesis Array C2 (4)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-ANG-2-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Angiogenesis Antibody Array 2 Kit. Detects 23 Human Angiogenic Factors. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
23
Target Names
Angiopoietin-1, Angiopoietin-2, Angiostatin, Endostatin, GCSF, GM-CSF, I-309 (TCA-3/CCL1), IL-10, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, I-TAC (CXCL11), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MMP-1, MMP-9, PECAM-1 (CD31), Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Targets Detected
Angiopoietin-1, Angiopoietin-2, Angiostatin, CCL1, CCL13, CCL7, CD31, CXCL11, Endostatin, G-CSF, GM-CSF, I-309, I-TAC, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-2, IL-4, MARC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TCA-3, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array C2 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Moon MH, Kim SY, Kim YJ, Kim SJ, et al. Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Postnatal Neovascularization in a Mouse Model of Hindlimb Ischemia. Cell Physiol Biochem. 2006, 17:279-290.
2. Raghu H., Lakka S., Gondi C., Mohanam S., et al. Suppression of uPA and uPAR Attenuates Angiogenin Mediated Angiogenesis in Endothelial and Glioblastoma Cell Lines. PLOS One, Published: August 27, 2010. DOI: 10.1371/journal.pone.0012458
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 468,51 €
Preis: 468,51 €
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