Hersteller |
Raybiotech
|
Kategorie |
|
Typ |
Assay |
Specific against |
Human |
Menge |
8 Sample Kit |
ArtNr |
AAH-ANG-G1-8 |
Targets |
ANG ;; IL6 ;; LEP ;; PGF ;;CCL2;;CCL5;;CXCL8;;FOXD3;;TGFB1;;THPO;;TIMP1;;VEGFA;;VEGFD |
eClass 6.1 |
32161000 |
eClass 9.0 |
32161000 |
Lieferbar |
|
Short description |
RayBio G-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types. |
Features |
- High sensitivity and specificity
- Low sample volume (10-100 ul per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
|
Number of Targets Detected |
20 |
Target Names |
Angiogenin, EGF, ENA-78 (CXCL5), bFGF, GRO alpha/beta/gamma, IFN-gamma, IGF-1, IL-6, IL-8 (CXCL8), Leptin, MCP-1 (CCL2), PDGF-BB, PLGF, RANTES (CCL5), TGF beta 1, TIMP-1, TIMP-2, Thrombopoietin (TPO), VEGF-A, VEGF-D |
Targets Detected |
Angiogenin, bFGF, CCL2, CCL5, CXCL5, CXCL8, EGF, ENA-78, GRO, IFN-gamma, IGF-1, IL-6, IL-8, Leptin, MCP-1, PDGF-BB, PlGF, RANTES, TGF beta 1, Thrombopoietin, TIMP-1, TIMP-2, TPO, VEGF-A, VEGF-D |
Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity |
Kit Components |
- Human Angiogenesis Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
|
Other Materials Required |
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Glass Slide |
Design Principle |
Sandwich-based |
Result Output |
Semi-Quantitative |
Detection Method |
Fluorescence Laser Scanner |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Citation |
Jose S., Hughbanks ML., Binder BY., Ingvale GC., Leach JK. Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels. Acta Biomater. 2014 May, 10(5):1955-64. doi: 10.1016/j.actbio.2014.01.020 |
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