Human Angiogenesis Array G2 (4)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-ANG-G2-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Human Angiogenesis Antibody Array 2 Kit. Detects 23 Human Angiogenic Factors. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
23
Target Names
Angiopoietin-1, Angiopoietin-2, Angiostatin, Endostatin, GCSF, GM-CSF, I-309 (TCA-3/CCL1), IL-10, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-4, I-TAC (CXCL11), MCP-3 (MARC/CCL7), MCP-4 (CCL13), MMP-1, MMP-9, PECAM-1 (CD31), Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Targets Detected
Angiopoietin-1, Angiopoietin-2, Angiostatin, CCL1, CCL13, CCL7, CD31, CXCL11, Endostatin, G-CSF, GM-CSF, I-309, I-TAC, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-2, IL-4, MARC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, TCA-3, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Angiogenesis Antibody Array G2 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
Blansfield JA, Caragacianu D, Alexander RA III, Tangrea MA, Morita SY, et al. Combining Agents that Target the Tumor Microenvironment Improves the Efficacy of Anticancer Therapy. Clin Cancer Res. 2008, 14(1):270–280.
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 584,44 €
Preis: 584,44 €
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