Human Apoptosis Array C1 (8)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 8 Sample Kit
ArtNr AAH-APO-1-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Secondary categories
Products, Functional Assays, Apoptosis Assays
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
43
Target Names
bad, bax, bcl-2, bcl-w, BID, BIM, Caspase-3, Caspase-8, CD40 (TNFRSF5), CD40 Ligand (TNFSF5), cIAP-2, Cytochrome C, DR6 (TNFRSF21), Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), HSP27, HSP60, HSP70, HTRA2, IGF-1, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGF-1 R, livin, p21, p27, p53, SMAC, Survivin (BIRC5), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNF alpha, TNF beta (TNFSF1B), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), XIAP
Targets Detected
4-1BB, Apo-1, bad, bax, bcl-2, bcl-w, BID, BIM, BIRC5, Caspase-3, Caspase-8, CD40, CD40 Ligand, cIAP-2, Cytochrome C, DR4, DR5, DR6, Fas, Fas Ligand, HSP27, HSP60, HSP70, HTRA, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, Livin, p21, p27, p53, SMAC, Survivin, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10A, TNFRSF10B, TNFRSF10D, TNFRSF21, TNFRSF5, TNFRSF6, TNFRSF9, TNFSF1B, TNFSF5, TNFSF6, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, XIAP
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Spadavecchia S, Gonzalez-Lopez OD, Kyla Driscoll Carroll KD, Diana Palmeri D, Lukac DM. Convergence of KSHV Reactivation with EBV latency and cellular growth mediated by the Notch signaling pathway in co-infected cells. J Virol. 2010:84(20):10488–15000.
2. Malla R., et al. Downregulation of uPAR and Cathepsin B Induces Apoptosis via Regulation of Bcl-2 and Bax and Inhibition of the PI3K/Akt Pathway in Gliomas. PLOS One. Published: October 29, 2010 DOI: 10.1371/journal.pone.0013731
3. Rahabi-Layachi H., et al. You have full text access to this content Distinct effects of inorganic phosphate on cell cycle and apoptosis in human vascular smooth muscle cells. J of Cell Phys. Accepted manuscript online: 27 JUN 2014. | DOI: 10.1002/jcp.24715
4. Wu J., et al. Blocking the bFGF/STAT3 interaction through specific signaling pathways induces apoptosis in glioblastoma cells. J Neurooncol publihsed online July 2014. DOI 10.1007/s11060-014-1529-8
5. Lattuada D., et al. Proapoptotic Activity of a Monomeric Smac Mimetic on Human Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis. Inflammation, Sept 2014, epub ahead of print. DOI: 10.1007/s10753-014-0012-1
6. Paydar, M., Kamalidehghan, B. et al. evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models. Drug Design, Development and Therapy, Volume 2014:8 Pages 719 - 733. June 2014
7. Karimian H., et al. Ferulago angulata activates intrinsic pathway of apoptosis in MCF-7 cells associated with G1 cell cycle arrest via involvement of p21/p27. Drug Design, Development and Therapy, Sept 2014, epub ahead of print.http://dx.doi.org/10.2147/DDDT.S68818

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Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 870,69 €
Preis: 870,69 €
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