Human Cytokine Array C1000 (2)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 2 Sample Kit
ArtNr AAH-CYT-1000-2
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array C6 & C7. Detects 120 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
120
Target Names
Adiponectin (ACRP30), AgRP, Amphiregulin, Angiogenin, Angiopoietin-2, Axl, BDNF, bFGF, BLC (CXCL13), BMP-4, BMP-6, beta-NGF, Betacellulin (BTC), CCL28 (MEC), CK beta 8-1 (CCL23), CNTF, CTACK (CCL27), Dtk, EGF, EGFR, ENA-78 (CXCL5), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), Fas (TNFRSF6/Apo-1), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GCSF, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-3 (CD50), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17A, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), I-TAC (CXCL11), Leptin, Light (TNFSF14), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NT-3, NT-4, Oncostatin M, Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-BB, PLGF, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), gp130, TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TARC (CCL17), TECK (CCL25), TGF beta 3, TGF beta 1, Thrombopoietin (TPO), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), uPAR, VEGF-A, VEGF-D
Targets Detected
ACRP30, Adiponectin, AgRP, Amphiregulin, Angiogenin, Angiopoietin-2, Apo-1, AXL, BDNF, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-6, BTC, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD50, CD54, CK beta 8-1, CNTF, CTACK, CX3CL1, CXCL1, CXCL11, CXCL12 alpha, CXCL13, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, Dtk, EGF, EGFR, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, ErbB1, Fas, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, HCC-4, HGF, I-309, I-TAC, ICAM-1, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R4, IL-1 Ra, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8, KGF, Leptin, LIGHT, Lymphotactin, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MPIF-2, MSP alpha/beta, NAP-2, NT-3, NT-4, Oncostatin M, Osteoprotegerin, PARC, PDGF-BB, PlGF, PPBP, RANTES, SCF, SDF-1 alpha, ST2, TARC, TCA-3, TECK, TGF beta 1, TGF beta 3, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10D, TNFRSF11B, TNFRSF18, TNFRSF6, TNFSF14, TNFSF18, TNFSF1B, TPO, TRAIL R3, TRAIL R4, uPAR, VEGF-A, VEGF-D, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
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2. Ishibashi Y, Sugita T, Nishikawa A. Cytokine secretion pro¢le of human keratinocytes exposed to Malassezia yeasts.FEMS Immunol Med Microbiol.2006(48)400–409
3. Celis JE, Moreira JMA, Gromova I, Cabezon T, et al. Towards discovery-driven translational research in breast cancer. FEBS J. 2004, 272:2-15.
4. Falkensammer C, Johrer K, Gander H, Ramoner R, Putz T, et al.IL-4 inhibits the TNF-a induced proliferation of renal cell carcinoma (RCC) and cooperates with TNF-a to induce apoptotic and cytokine responses by RCC: implications for antitumor immune responses. Cancer Immunol Immunother.2006, 55: 1228–1237 DOI 10.1007/s00262-006-0122-1
5. Dietze-Schroeder D, Sell H, Uhlig M, Koenen M, Eckel J. Autocrine Action of Adiponectin on Human Fat Cells Prevents the Release of Insulin Resistance-Inducing Factors. Diabetes. 2005, 54:2003-2011.
6. Klimcakova E, Moro C, Mazzucotelli A, Lolmede K, Viguerie N, et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. Biochem Biophys Res Comm.2007, 358(3):897–902
7. Moro C, Klimeakova E, Lolmede K, Berlan M, et al. Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia. 2007, 50:1038–1047.
8. Wang WE, Yang D, Li L, et al. PHD2 Silencing Enhances the Survival and Paracrine Function of Transplanted Adipose-Derived Stem Cells in Infarcted Myocardium. Circ. Res. 2013 May 21.
9. Wang Y, Cai D, Brendel C, Barett C, Erben P, et al. Adaptive secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates imatinib and nilotinib resistance in BCR/ABL+ progenitors via JAK-1/STAT-5 pathway activation. Blood. 2007, 109:2147–2155.
10. Giroux M, Denis F. Human CD1d-unrestricted NKT cells release chemokines upon Fas engagement. Blood. 2005, 105(2):703–710.
11. Hartiala P, Hytonen J, Pelkonen J, Kimppa K, et al. Transcriptional response of human dendritic cells to Borrelia garinii---defective CD38 and CCR7 expression detected. J Leuk Biol. 2007, 82:33-43.
12. The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner
13. Ancuta P, Wang J, Gabuzda D. CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interacting with CX3CL1-expressing endothelial cells. J Leuk Biol. 2006, 80:1156-1164.
14. Maury E, Ehala-Aleksejev K, Guiot Y, Detry R, Vandenhooft A, Brichard SM. Adipokines oversecreted by omental adipose tissue in human obesity. Am J Physiol Endocrinol Metab. 2007, 293:E656–E665.
15. Henrichot E, Juge-Aubry CE, Pernin A, Pache J-C, et al. Production of Chemokines by Perivascular Adipose Tissue: A Role in the Pathogenesis of Atherosclerosis? Aterioscler Thromb Vasc Biol. 2005, 25:2594-2599
16. Fu SY, Su GY, McKinley SH, Yen MT. Cytokine expression in pediatric subperiosteal orbital abscesses. Can J Ophthalmol. 2007, 42:865-869.
17. Celis JE, Moreira JMA, Gromova I, Cabezon T, et al. Towards discovery-driven translational research in breast cancer. FEBS J. 2005, 272:
18. Celis JE, Moreira JMA, Cabezon T, Gromov P, et al. Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients: Toward Dissecting The Molecular Circuitry of Epithelial-Adipocyte Stromal Cell Interactions. Mol Cell Proteomics. 2005, 4:492-522.
19. Celis JE, Gromov P, Moreira JMA, Cabezon T, et al. Apocrine Cysts of the Breast: Biomarkers, Origin, Enlargement, and Relation with Cancer Phenotype. Mol Cell Proteomics. 2006, 5:432-483.
20. Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, et al. Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins. Nat Med. 2007, 13(11):1359–1362.
21. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007, 53:42–52.
22. Zhang X, Jin J P, Tang Y N, Speer D, Sujkowska D, et al. IFN-1a Inhibits the Secretion of Th17-Polarizing Cytokines in Human Dendritic Cells via TLR7 Up-Regulation.J. Immunol. 2009, 182:3928–3936
23. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007, 53:42–52.
24. Osman L, Yacoub MH, Latif N, Amrani M, Chester AH. Role of Human Valve Interstitial Cells in Valve Calcification and Their Response to Atorvastatin. Circulation. 2006, 114(suppl.1):I547–I552.
25. Lisco A, Grivel JC, Biancotto A, Vanpouille C, et al. Viral Interactions in Human Lymphoid TissueL Human Herpesvirus 7 Suppresses the replication of CCF5-Tropic Human Immunodeficiency Virus Type 1 via CD4 Modulation. J Virol. 2007, 81(2):708–717.
26. Klimcakova E, Moro C, Mazzucotelli A, Lolmede K, Viguerie N, et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. BBRC.2007, 358(3):897-902
27. Klimcakova E, Moro C, Mazzucotelli A, Lolmede K, Viguerie N, et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. BBRC.2007, 358(3):897-902
28. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005, 16:256–265.
29. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008, 102: 77-85
30. Skopkova M, Penesova A, Sell H, Radikova Z, Vlcek M, et al. Protein Array Reveals Differentially Expressed Proteins in Subcutaneous Adipose Tissue in Obesity.Obesity (Silver Spring). 2007, 15(10):2396–2406.
31. Giroux, M et al. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement. Blood Volume 105, Issue 2, Pages 703-10, Jan 2005
32. Shih Y., Wang M., Zhou J., Peng H., Lee D., Chiu J. Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21. Gut 2014 Epub ahead of print June 17 2014, doi:10.1136/gutjnl-2013-306302
33. Boucek RJ., et al. < i> Ex Vivo Paracrine Properties of Cardiac Tissue: Effects of Chronic Heart Failure. J of Heart and Lung Transpl. Available online 17 July 2014 DOI: 10.1016/j.healun.2014.07.010
34. Chung E., et al. CXCL5 abundant in the wound fluid at the late phase of wound healing, possibly promoting migration of mesenchymal stem cells and vascular tube formation. Tissue Engineering and Regenerative Medicine August 2014, Volume 11, Issue 4, pp 317-322
35. Lee YC., et al. BMP4 Promotes Prostate Tumor Growth in Bone through Osteogenesis. Cancer Research 71(15), 5194–203. DOI: 10.1158/0008-5472.CAN-10-4374
36. Kavitha, C. V., Deep, G., Gangar, S. C., Jain, A. K., Agarwal, C. and Agarwal, R. (2014), Silibinin inhibits prostate cancer cells- and RANKL-induced osteoclastogenesis by targeting NFATc1, NF-kappaB, and AP-1 activation in RAW264.7 cells. Mol. Carcinog., 53: 169–180. doi: 10.1002/mc.21959
37. Gromov P., et al. Tumor interstitial fluid — A treasure trove of cancer biomarkers. Biochimica et Biophysica Acta 1834 (2013) 2259–2270. http://dx.doi.org/10.1016/j.bbapap.2013.01.013
38. Lin Y., et al. A panel of four cytokines predicts the prognosis of patients with malignant gliomas. J Neurooncol (2013) 114:199–208, DOI 10.1007/s11060-013-1171-x
39. Zhang Y, Liu H, Wang L, Yang F, Hu Y, et al. (2013) Comparative Study of the Cytokine/Chemokine Response in Children with Differing Disease Severity in Enterovirus 71-Induced Hand, Foot, and Mouth Disease. PLoS ONE 8(6): e67430. doi:10.1371/journal.pone.0067430
40. Hastie A., et al. Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes . Journal of Allergy and Clinical Immunology, Volume 125, Issue 5, May 2010, Pages 1028–1036.e13. DOI:10.1016/j.jaci.2010.02.008

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Menge: 2 Sample Kit
Lieferbar: In stock
Listenpreis: 704,05 €
Preis: 704,05 €
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