Human Cytokine Array C2000 (2)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 2 Sample Kit
ArtNr AAH-CYT-2000-2
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
120
Target Names
Adiponectin (ACRP30), Activin A, AgRP, ALCAM (CD166), Amphiregulin, Angiogenin, Angiopoietin-2, Axl, CD80 (B7-1), BDNF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-6, BMP-7, beta-NGF, Betacellulin (BTC), Cardiotrophin-1 (CT-1), CCL28 (MEC), CD14, Ck beta 8-1 (CCL23), CNTF, CTACK (CCL27), CXCL16, DR6 (TNFRSF21), Dtk, EGF, EGFR, ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB3, E-Selectin, Fas Ligand (TNFSF6), Fas Ligand (TNFSF6), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GCSF, GDNF, GITR (TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), HCC-4 (CCL16), HGF, I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, IL-1 R2, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15, IL-16, IL-17A, IL-18 BP alpha, IL-18 R beta (AcPL), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-2 R alpha, IL-21 R, IL-3, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1, Leptin, Leptin R, LIF, Light (TNFSF14), L-Selectin (CD62L), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NT-3, NT-4, Oncostatin M, Osteoprotegerin (TNFRSF11B), PARC (CCL18), PDGF-AA, PDGF R alpha, PDGF R beta, PDGF-AB, PDGF-BB, PECAM-1 (CD31), PLGF, Prolactin, RANTES (CCL5), SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), gp130, Siglec-5 (CD170), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TARC (CCL17), TECK (CCL25), TGF beta 2, TGF alpha, TGF beta 3, TGF beta 1, Thrombopoietin (TPO), Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta (TNFSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), uPAR, VE-Cadherin (CDH5), VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected
AcPL, ACRP30, Activin A, Adiponectin, AgRP, ALCAM, Amphiregulin, Angiogenin, Angiopoietin-2, Apo-1, AXL, B7-1, BDNF, beta-NGF, Betacellulin, bFGF, BLC, BMP-4, BMP-5, BMP-6, BMP-7, BTC, c-kit, Cardiotrophin-1, CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL7, CCL8, CD102, CD105, CD117, CD122, CD14, CD170, CD31, CD50, CD54, CD62L, CD80, CK beta 8-1, CNTF, Common gamma Chain, CT-1, CTACK, CX3CL1, CXCL1, CXCL10, CXCL11, CXCL12 alpha, CXCL12 beta, CXCL13, CXCL16, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, DR6, Dtk, E-Selectin, EGF, EGFR, ENA-78, Endoglin, Eotaxin-1, Eotaxin-2, Eotaxin-3, ErbB1, ErbB3, Fas, Fas Ligand, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GITR, GITR Ligand, GM-CSF, gp130, GRO, GRO alpha, HCC-4, HGF, I-309, I-TAC, ICAM-1, ICAM-2, ICAM-3, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 R1, IL-1 R2, IL-1 R4, IL-1 Ra, IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 2, IL-15, IL-16, IL-17, IL-18 BP alpha, IL-18 R beta, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-21 R, IL-3, IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8, IL-9, IP-10, KGF, L-Selectin, LAP/TGF beta 1, Leptin, Leptin R, LIF, LIGHT, Lymphotactin, M-CSF, M-CSF R, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MEC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-13, MMP-3, MMP-9, MPIF-1, MPIF-2, MSP alpha/beta, NAP-2, NGFR, NT-3, NT-4, Oncostatin M, Osteoprotegerin, PARC, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, PlGF, PPBP, Prolactin, RANTES, SCF, SCF R, SDF-1 alpha, SDF-1 beta, Siglec-5, ST2, TARC, TCA-3, TECK, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-4, TNF alpha, TNF beta, TNF RI, TNF RII, TNFRSF10D, TNFRSF11B, TNFRSF16, TNFRSF18, TNFRSF21, TNFRSF6, TNFSF14, TNFSF18, TNFSF1B, TNFSF6, TPO, TRAIL R3, TRAIL R4, uPAR, VE-Cadherin, VEGF-A, VEGF-D, VEGFR2, VEGFR3, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C2000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Yang CC, Shih YH, Ko MH, Hsu SY, Cheng H, et al. Transplantation of Human Umbilical Mesenchymal Stem Cells from Wharton’s Jelly after Complete Transection of the Rat Spinal Cord. PLoS ONE. 2008, 3(10):e3336. doi:10.1371/journal.pone.0003336
2. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis
3. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013, 73:662–671.
4. Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007, 67(8):3646–3653.
5. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007, 2(9): e941. doi:10.1371/journal.pone.0000941
6. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M, et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008, 93: 2255–2262
7. Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006, 26(5):483-490.
8. Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007, 81(14):7584–7597.
9. Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007, 81(14):7584–7597.
10. Shimizu, Masaki, Mondo Kuroda, and Michio Konishi. "Extensive Serum Biomarker Analysis in Patients with Enterohemorrhagic Escherichia Coli O111-induced Hemolytic-uremic Syndrome." Cytokine. ScienceDirect, Mar. 2014. Web.
11. Fujita K, Ewing C M , Sokoll L J, Elliott D J, Cunningham M, et al.Cytokine Profiling of Prostatic Fluid From Cancerous Prostate Glands Identifies Cytokines Associated With Extent of Tumor and Inflammation. Prostate. 2008, 68:872–882.
12. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis
13. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008, 8:e18.
14. Wang T, Wang YY, Zhou R, et al. Bioactive proteins in healthy pregnancies and preeclampsia: relevance to hypertension and proteinuria. Chin Med J. 2013, 126(11):2015–2020.
15. Wang T, Wang YY, Zhou R, et al. Bioactive proteins in healthy pregnancies and preeclampsia: relevance to hypertension and proteinuria. Chin Med J. 2013, 126(11):2015–2020.
16. Belletti B, Vaidya J, Andrea S, Entschladen F, Roncadin M, et al. Targeted Intraoperative Radiotherapy Impairs the Stimulation of Breast Cancer Cell Proliferation and Invasion Caused by Surgical Wounding. Clin Cancer Res March 1, 2008 14, 1325
17. Belletti B, Vaidya J, Andrea S, Entschladen F, Roncadin M, et al. Targeted Intraoperative Radiotherapy Impairs the Stimulation of Breast Cancer Cell Proliferation and Invasion Caused by Surgical Wounding. Clin Cancer Res March 1, 2008 14, 1325
18. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Published Online First August 4, 2014, doi: 10.1158/1541-7786.MCR-14-0159
19. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 – 1883

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 2 Sample Kit
Lieferbar: In stock
Listenpreis: 1.422,01 €
Preis: 1.422,01 €
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