Human Cytokine Array C3 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-3-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
42
Target Names
ENA-78 (CXCL5), GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 delta (CCL15), RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), EGF, IGF-1, Angiogenin, Oncostatin M, Thrombopoietin (TPO), VEGF-A, PDGF-BB, Leptin
Targets Detected
Angiogenin, CCL1, CCL15, CCL17, CCL2, CCL22, CCL5, CCL7, CCL8, CXCL1, CXCL12 alpha, CXCL8, CXCL9, EGF, ENA-78, G-CSF, GM-CSF, GRO, GRO alpha, I-309, IFN-gamma, IGF-1, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, Leptin, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MDC, MIG, MIP-1 delta, Oncostatin M, PDGF-BB, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TGF beta 1, Thrombopoietin, TNF alpha, TNF beta, TNFSF1B, TPO, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
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5. Wang S, Yang J, Qian J, Wezeman M, Kwak LW, Yi Q. Tumor evasion of the immune system: inhibiting p38 MAPK signaling restores the function of dendritic cells in multiple myeloma. Blood. 2006, 107:2431-2439.
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20. Benitez S, Camacho M, Bancells C, Vila L, Sanchez-Quesada JL, Ordonez-Llanos J. Wide proinflammatory effect of electronegative low-density lipoprotein on human endothelial cells assayed by a protein array. Biochim Biophys Acta. 2006, 1761:1014-1021.
21. Ryzhov S, Goldstein AE, Matafonov A, Zeng D, Biaggioni I, Feoktistov I. Adenosine-Activated Mast Cells Induce IgE Synthesis by B Lymphocytes: An A(2B)-Mediated Process Involving Th2 Cytokines IL-4 and IL-13 with Implications for Asthma. J Immunol. 2004, 172:7726-7733.
22. Tang X, Marciano DL, Leeman SE, Amar S. LPS induces the interaction of a transcription factor, LPS-induced TNF-alpha factor, and STAT6(B) with effects on multiple cytokines. PNAS. 2005, 102(14):5132-5137.
23. Patrone JB, Bish SE, Stein DC. TNF-alpha-Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response. J Immunol. 2006, 177:1314-1322.
24. Gogolak P, Rethi B, Szatmari I, Lanyi A, et al. Differentiation of CD1a- and CD1a+ monocyte-derived dendritic cells is biased by lipid environment and PPAR-gamma. Blood. 2007, 109:643-652.
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27. Robson RL, Reed NA, Horvat RT. Differential activation of inflammatory pathways in A549 type II pneumocytes by Streptococcus pneumoniae strains with different adherence properties. BMC Infect Dis. 2006, 6:71. Available at http://www.biomedcentral.com/1471-2334/6/71. Last accessed Jan 25, 2008.
28. Bogdanski D, Esenwein SA, Prymak O, Epple M, Muhr G, Koller M. Release of Leucocyte Mediators after Contact to Coated and Non-coated NITI-SMA. Biomedizinische Technik Jg. 2004, 49:562-563.
29. Ritter M, Mennerich D, Weith A, Seither P. Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response. J Inflamm. 2005, 2:16. Available at: http://www.journal-inflammation.com/content/2/1/16. Last accessed Jan 25, 2008.
30. Tay SS, McCormack A, Rose ML. Effect of Cognate Human CD4+ T Cell and Endothelial Cell Interactions Upon Chemokine Production. Transplant. 2004, 78(7):987-994.
31. Duenas AI, Aceves M, Orduna A, Diaz R, Crespo MS, Garcia-Rodriguez C. Francisella tularensis LPS induces the production of cytokines in human monocytes and signals via Toll-like receptor 4 with much lower potency than E. coli LPS. Intl Immunol. 2006, 18(5):785-795.
32. Marks DJB, Harbord MWN, MacAllister R, Rahman FZ, Young J, et al. Defective acute inflammation in Crohn's disease: a clinical investigation. Lancet. 2006, 367:668-678.
33. Nick JA, Coldren CD, Geraci MA, Poch KR, et al. Recombinant human activated protein C reduces human endotoxin–induced pulmonary inflammation via inhibition of neutrophil chemotaxis. Blood. 2004, 104(13):3878-3885.
34. Kaplin AI, Deshpande DM, Scott E, Krishnan C, Carmen JS, et al. IL-6 induces regionally selective spinal cord injury in patients with the neuroinflammatory disorder transverse myelitis. J Clin Invest. 2005, 115:2731-2741.
35. Nichols MT, Gidey E, Matzakos T, Dahl R, et al. Secretion of Cytokines and Growth Factors Into Autosomal Dominant Polycystic Kidney Disease Liver Cyst Fluid. Hepatology. 2004, 40:836-846.
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38. Vasquez-martin A, Colomer R, Menendez JA. Protein array technology to detect HER2 (erbB-2)-induced 'cytokine signature' in breast cancer. Eur J Cancer. 2007, 43:1117–1124.
39. Patrone J, Bish S, Stein D. TNF-alpha -Independent IL-8 Expression: Alterations in Bacterial Challenge Dose Cause Differential Human Monocytic Cytokine Response.J Immunol. 2006, 177:1314–1322.
40. De Ceuninck F, Marcheteau E, Berger S, Caliez A, et al. Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome. J Biomol Tech. 2005, 16:256–265.
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Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 667,40 €
Preis: 667,40 €
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