Human Cytokine Array C5 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-5-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
80
Target Names
ENA-78 (CXCL5), GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), EGF, IGF-1, Angiogenin, Oncostatin M, Thrombopoietin (TPO), VEGF-A, PDGF-BB, Leptin, BDNF, BLC (CXCL13), Ck beta 8-1 (CCL23), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10 (CXCL10), LIF, Light (TNFSF14), MCP-4 (CCL13), MIF, MIP-3 alpha (CCL20), NAP-2 (PPBP/CXCL7), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PARC (CCL18), PLGF, TGF beta 2, TGF beta 3, TIMP-1, TIMP-2
Targets Detected
Angiogenin, BDNF, BLC, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20, CCL22, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CK beta 8-1, CX3CL1, CXCL1, CXCL10, CXCL12 alpha, CXCL13, CXCL6, CXCL7, CXCL8, CXCL9, EGF, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GM-CSF, GRO, GRO alpha, HGF, I-309, IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-16, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, KGF, Leptin, LIF, LIGHT, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIF, MIG, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MPIF-2, NAP-2, NT-3, NT-4, Oncostatin M, Osteopontin, Osteoprotegerin, PARC, PDGF-BB, PlGF, PPBP, RANTES, SCF, SDF-1 alpha, SPP1, TARC, TCA-3, TCK-1, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNFRSF11B, TNFSF14, TNFSF1B, TPO, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
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Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 667,40 €
Preis: 667,40 €
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