Human Cytokine Array C6 (2)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 2 Sample Kit
ArtNr AAH-CYT-6-2
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
60
Target Names
Angiogenin, BDNF, BLC (CXCL13), BMP-4, BMP-6, Ck beta 8-1 (CCL23), CNTF, EGF, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-6, FGF-7 (KGF), Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GDNF, GM-CSF, I-309 (TCA-3/CCL1), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-4, IGF-1, IL-10, IL-13, IL-15, IL-16, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, Leptin, Light (TNFSF14), MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), NAP-2 (PPBP/CXCL7), NT-3, PARC (CCL18), PDGF-BB, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TGF beta 3, TNF alpha, TNF beta (TNFSF1B)
Targets Detected
Angiogenin, BDNF, BLC, BMP-4, BMP-6, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20, CCL22, CCL23, CCL24, CCL26, CCL5, CCL7, CCL8, CK beta 8-1, CNTF, CX3CL1, CXCL12 alpha, CXCL13, CXCL6, CXCL7, CXCL9, EGF, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-6, FGF-7, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, GM-CSF, I-309, IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-4, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-1 F3, IL-1 Ra, IL-10, IL-13, IL-15, IL-16, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, KGF, Leptin, LIGHT, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIG, MIP-1 delta, MIP-3 alpha, MPIF-2, NAP-2, NT-3, PARC, PDGF-BB, PPBP, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TCK-1, TGF beta 1, TGF beta 3, TNF alpha, TNF beta, TNFSF14, TNFSF1B
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Henrichot E, Juge-Aubry CE, Pernin A, Pache J-C, et al. Production of Chemokines by Perivascular Adipose Tissue: A Role in the Pathogenesis of Atherosclerosis? Aterioscler Thromb Vasc Biol. 2005, 25:2594-2599
2. Kim D, Cho H, Yang S, Shin J, Huh C, et al.IGFBP-2 Contributes to the Proliferation of Less Proliferative Cells in Forming Skin Equivalents.Tissue Eng Part A. 2009, 15(5):1075–1080.
3. Falkensammer C, Johrer K, Gander H, Ramoner R, Putz T, et al.IL-4 inhibits the TNF-a induced proliferation of renal cell carcinoma (RCC) and cooperates with TNF-a to induce apoptotic and cytokine responses by RCC: implications for antitumor immune responses. Cancer Immunol Immunother.2006, 55: 1228–1237 DOI 10.1007/s00262-006-0122-1
4. Dietze-Schroeder D, Sell H, Uhlig M, Koenen M, Eckel J. Autocrine Action of Adiponectin on Human Fat Cells Prevents the Release of Insulin Resistance-Inducing Factors. Diabetes. 2005, 54:2003-2011.
5. Klimcakova E, Moro C, Mazzucotelli A, Lolmede K, Viguerie N, et al.Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. Biochem Biophys Res Comm.2007, 358(3):897–902
6. Moro C, Klimeakova E, Lolmede K, Berlan M, et al. Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia. 2007, 50:1038–1047.
7. Wang WE, Yang D, Li L, et al. PHD2 Silencing Enhances the Survival and Paracrine Function of Transplanted Adipose-Derived Stem Cells in Infarcted Myocardium. Circ. Res. 2013 May 21.
8. Wang Y, Cai D, Brendel C, Barett C, Erben P, et al. Adaptive secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates imatinib and nilotinib resistance in BCR/ABL+ progenitors via JAK-1/STAT-5 pathway activation. Blood. 2007, 109:2147–2155.
9. Giroux M, Denis F. Human CD1d-unrestricted NKT cells release chemokines upon Fas engagement. Blood. 2005, 105(2):703–710.
10. Hartiala P, Hytonen J, Pelkonen J, Kimppa K, et al. Transcriptional response of human dendritic cells to Borrelia garinii---defective CD38 and CCR7 expression detected. J Leuk Biol. 2007, 82:33-43.
11. Human Bone Marrow-Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis
12. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013, 73:662–671.
13. Ancuta P, Wang J, Gabuzda D. CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interacting with CX3CL1-expressing endothelial cells. J Leuk Biol. 2006, 80:1156-1164.
14. Maury E, Ehala-Aleksejev K, Guiot Y, Detry R, Vandenhooft A, Brichard SM. Adipokines oversecreted by omental adipose tissue in human obesity. Am J Physiol Endocrinol Metab. 2007, 293:E656–E665.
15. Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1. PLoS One. 2013, 8(1): e55199.
16. Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007, 67(8):3646–3653.
17. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007, 2(9): e941. doi:10.1371/journal.pone.0000941
18. Fu SY, Su GY, McKinley SH, Yen MT. Cytokine expression in pediatric subperiosteal orbital abscesses. Can J Ophthalmol. 2007, 42:865-869.
19. Celis JE, Moreira JMA, Gromova I, Cabezon T, et al. Towards discovery-driven translational research in breast cancer. FEBS J. 2005, 272:
20. Celis JE, Moreira JMA, Cabezon T, Gromov P, et al. Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients: Toward Dissecting The Molecular Circuitry of Epithelial-Adipocyte Stromal Cell Interactions. Mol Cell Proteomics. 2005, 4:492-522.
21. Celis JE, Gromov P, Moreira JMA, Cabezon T, et al. Apocrine Cysts of the Breast: Biomarkers, Origin, Enlargement, and Relation with Cancer Phenotype. Mol Cell Proteomics. 2006, 5:432-483.
22. Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, et al. Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins. Nat Med. 2007, 13(11):1359–1362.
23. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007, 53:42–52.
24. Zhang X, Jin J P, Tang Y N, Speer D, Sujkowska D, et al. IFN-1a Inhibits the Secretion of Th17-Polarizing Cytokines in Human Dendritic Cells via TLR7 Up-Regulation.J. Immunol. 2009, 182:3928–3936
25. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007, 53:42–52.
26. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M, et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008, 93: 2255–2262
27. Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006, 26(5):483-490.
28. Meltzer K, Standley P.Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion.J Am Osteopath Assoc. 2007, 107:527–536
29. Bhattacharyya S, Borthakur A, P Dudeja, Tobacman J. Carrageenan Reduces Bone Morphogenetic Protein-4 (BMP4) and Activates the Wnt/beta-Catenin Pathway in Normal Human Colonocytes.Dig Dis Sci, 2007, 52:2766–2774
30. He T, Peterson TE, Katusic ZS. Paracrine mitogenic effect of human endothelial progenitor cells: role of interleukin-8. Am J Physiol Heart Circ Physiol. 2005, 289:H968-H972.
31. Hausman GJ, Poulos SP, Richardson RL, Barb CR, et al. Secreted proteins and genes in fetal and neonatal pig adipose tissue and stromal-vascular cells. J Animal Sci. 2006, 84(7):1666–1681.
32. Nordstrom T, Jendholm J, Samuelsson M, Forsgren A, Riesback K. The IgD-binding domain of the Morazella IgD-binding protein MID (MID 962-1200) activates human B cells in the presence of T cell cytokines. J Leuk Biol. 2006, 79:319–329.
33. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008, 8:e18.
34. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008, 102: 77-85
35. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
36. Shih Y., Wang M., Zhou J., Peng H., Lee D., Chiu J. Endothelial progenitors promote hepatocarcinoma intrahepatic metastasis through monocyte chemotactic protein-1 induction of microRNA-21. Gut 2014 Epub ahead of print June 17 2014, doi:10.1136/gutjnl-2013-306302
37. Boucek RJ., et al. < i> Ex Vivo Paracrine Properties of Cardiac Tissue: Effects of Chronic Heart Failure. J of Heart and Lung Transpl. Available online 17 July 2014 DOI: 10.1016/j.healun.2014.07.010
38. Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Published Online First August 4, 2014, doi: 10.1158/1541-7786.MCR-14-0159
39. Chung E., et al. CXCL5 abundant in the wound fluid at the late phase of wound healing, possibly promoting migration of mesenchymal stem cells and vascular tube formation. Tissue Engineering and Regenerative Medicine August 2014, Volume 11, Issue 4, pp 317-322
40. Pual B., et al. BRCA1-IRIS inactivation sensitizes ovarian tumors to cisplatin. Oncogene Sept 2014, 18 August 2014, doi:10.1038/onc.2014.237.
41. Lee YC., et al. BMP4 Promotes Prostate Tumor Growth in Bone through Osteogenesis. Cancer Research 71(15), 5194–203. DOI: 10.1158/0008-5472.CAN-10-4374
42. Deng W., et al. Down-modulation of TNFSF15 in ovarian cancer by VEGF and MCP-1 is a pre-requisite for tumor neovascularization. Angiogenesis (2012) 15:71–85 DOI 10.1007/s10456-011-9244-y
43. Zhang Y, Liu H, Wang L, Yang F, Hu Y, et al. (2013) Comparative Study of the Cytokine/Chemokine Response in Children with Differing Disease Severity in Enterovirus 71-Induced Hand, Foot, and Mouth Disease. PLoS ONE 8(6): e67430. doi:10.1371/journal.pone.0067430

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 2 Sample Kit
Lieferbar: In stock
Listenpreis: 472,99 €
Preis: 472,99 €
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