Human Cytokine Array C9 (2)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 2 Sample Kit
ArtNr AAH-CYT-9-2
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 9 Kit. Detects 51 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
51
Target Names
Adipsin (Complement Factor D), BCAM, CD30 (TNFRSF8), CD40 (TNFRSF5), Fc gamma RIIB/C (CD32B/C), Ferritin, FLRG, Follistatin, Furin, Galectin-7, GDF-15, Growth Hormone, IL-10 R alpha, IL-22, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-31, Insulin, Luteinizing hormone, LIMPII, LYVE-1, Marapsin, MICA, MICB, MMP-2, MMP-7, MMP-8, MMP-10, NCAM-1 (CD56), Nidogen-1, NrCAM, NRG1-beta 1 (HRG1-beta 1), Osteopontin (SPP1), PAI-1, Platelet factor 4 (CXCL4), PSA-total, RAGE, RANK (TNFRSF11A), Resistin, SAA (Serum Amyloid A), Siglec-9, TACE, TIM-1 (KIM-1), TRAIL R2 (TNFRSF10B/DR5), Trappin-2, TREM-1, TSH, TSLP, VCAM-1 (CD106), VEGF-C, XEDAR
Targets Detected
Adipsin, BCAM, CD106, CD30, CD32B/C, CD40, CD56, CXCL14, CXCL4, DR5, Fc gamma RIIB/C, Ferritin, FLRG, Follistatin, Furin, Galectin-7, GDF-15, Growth Hormone, IL-10 R alpha, IL-22, IL-28A, IL-29, IL-31, Insulin, KIM-1, LIMPII, Luteinizing hormone, LYVE-1, Marapsin, MICA, MICB, MMP-10, MMP-2, MMP-7, MMP-8, NCAM-1, Nidogen-1, NrCAM, NRG1-beta 1, Osteopontin, PAI-1, Platelet Factor 4, PSA-total, RAGE, RANK, Resistin, SAA, Serum Amyloid A, Siglec-9, SPP1, TACE, TIM-1, TNFRSF10B, TNFRSF11A, TNFRSF5, TNFRSF8, TRAIL R2, Trappin-2, TREM-1, TSH, TSLP, VCAM-1, VEGF-C, XEDAR
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C9 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009, 139(7):1315–1326.
Menge: 2 Sample Kit
Lieferbar: In stock
Listenpreis: 704,05 €
Preis: 704,05 €
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