Human Growth Factor Array C1 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-GF-1-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
41
Target Names
Amphiregulin, bFGF, beta-NGF, EGF, EGFR, FGF-4, FGF-6, FGF-7 (KGF), GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R (CD117/c-kit), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected
Amphiregulin, beta-NGF, bFGF, c-kit, CD117, EGF, EGFR, ErbB1, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, HB-EGF, HGF, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, KGF, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PlGF, SCF, SCF R, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGF-D, VEGFR2, VEGFR3
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Growth Factor Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Tanaka H., et al. Liver Condition Affects Bovine Oocyte Qualities by Changing the Characteristics of Follicular Fluid and Plasma. Reprod Domest Anim. 2013 Aug, 48(4):619-26. doi: 10.1111/rda.12135
2. Differential secretome analysis of cancer-associated fibroblasts and bone marrow-derived precursors to identify microenvironmental regulators of colon cancer progression
3. Christov C, Cretien F, Abou-Kalil R, Bassez G, Vallet G, et al. Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 2007, 18:1397–1409.
4. De S, Razorenova O, McCabe NP, O'Toole T, Qin J, Byzova TV. VEGF-integrin interplay controls tumor growth and vascularization. Proc Natl Acad Sci USA. 2005, 102(21):7589–7594.
5. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008, 8:e18.
6. Traktuev D, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks. Circulation Research. 2008, 102: 77-85
7. Okuda H., Kobayashi A., Xia B., Watabe M., et al. Hyaluronan Synthase HAS2 Promotes Tumor Progression in Bone by Stimulating the Interaction of Breast Cancer Stem–Like Cells with Macrophages and Stromal Cells. Cancer Research. January 15, 2012 72, 537
8. Al-aidaroos A., et al. Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 2013, 123(8):3459–3471. doi:10.1172/JCI66824.
9. Dilwali, S et al. Primary culture of human Schwann and schwannoma cells: Improved and simplified protocol. Hearing Research 2014. Epub Ahead of Print DOI: 10.1016/j.heares.2014.05.006
10. Glinskii OV, Huxley VH, Glinskii VV, Rubin LJ, Glinsky VV (2013) Pulsed Estrogen Therapy Prevents Post-OVX Porcine Dura Mater Microvascular Network Weakening via a PDGF-BB-Dependent Mechanism. PLoS ONE 8(12): e82900. doi:10.1371/journal.pone.0082900
Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 667,40 €
Preis: 667,40 €
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