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Human Growth Factor Array G1

Human Growth Factor Array G1

Hersteller	Raybiotech
Kategorie	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Cell Status Adenocarcinoma Mesothelioma Senescence Arrays By Organ Bladder Cancer Colorectal Cancer Neuronal Cell Markers Antibody Arrays
Typ	Array
Specific against	Human
Menge	4 Sample Kit
ArtNr	AAH-GF-G1-4
Targets	KDR ;; PGF ;;AREG;;FGF4;;FGF6;;FGF7;;HBEGF;;IGFBP1;;IGFBP2;;IGFBP3;;NTF4;;PDGFA;;TGFB1;;VEGFA;;VEGFD
eClass 6.1	32161000
eClass 9.0	32161000
Lieferbar
Short description	RayBio G-Series Human Growth Factor Antibody Array 1 Kit. Detects 41 Human Growth Factors. Suitable for all liquid sample types.
Features	High sensitivity and specificity Low sample volume (10-100 ul per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
Number of Targets Detected	41
Target Names	Amphiregulin, bFGF, beta-NGF, EGF, EGFR, FGF-4, FGF-6, FGF-7 (KGF), GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-1, IGF-1 R, IGF-2, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R (CD117/c-kit), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGFR2, VEGFR3, VEGF-D
Targets Detected	Amphiregulin, beta-NGF, bFGF, c-kit, CD117, EGF, EGFR, ErbB1, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, HB-EGF, HGF, IGF-1, IGF-1 R, IGF-2, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, KGF, M-CSF, M-CSF R, NT-3, NT-4, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PlGF, SCF, SCF R, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, VEGF-A, VEGF-D, VEGFR2, VEGFR3
Suggested Applications	Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components	Human Growth Factor Antibody Array G1 Slide(s) Blocking Buffer Wash Buffer 1 Wash Buffer 2 Biotinylated Detection Antibody Cocktail Streptavidin-Conjugated Fluor Lysis Buffer Adhesive Plastic Strips 30 ml Centrifuge Tube Manual
Other Materials Required	Small plastic boxes or containers Pipettors, pipette tips and other common lab consumables Orbital shaker or oscillating rocker Aluminum foil Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline	Dry the glass slide Block array surface Incubate with Sample Incubate with Biotinylated Detection Antibody Cocktail Incubate with Streptavidin-Conjugated Fluor Disassemble the glass slide Scan with a gene microarray laser scanner Perform densitometry and analysis
Storage	For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support	Glass Slide
Design Principle	Sandwich-based
Result Output	Semi-Quantitative
Detection Method	Fluorescence Laser Scanner
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation	1. Zhang M., Xu M., Zhou Z., Zhou J., Zhao Y., Wang Z., Lu M. The Differentiation of Human Adipose-Derived Stem Cells towards a Urothelium-Like Phenotype In Vitro and the Dynamic Temporal Changes of Related Cytokines by Both Paracrine and Autocrine Signal Regulation. PLOS One, Published: April 21, 2014, DOI: 10.1371/journal.pone.0095583 [Epub ahead of print] 2. Christov C, Cretien F, Abou-Kalil R, Bassez G, Vallet G, et al. Muscle Satellite Cells and Endothelial Cells: Close Neighbors and Privileged Partners. Mol Biol Cell. 2007, 18:1397–1409.

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