Hersteller |
Raybiotech
|
Kategorie |
|
Typ |
Array |
Specific against |
Human |
Menge |
8 Sample Kit |
ArtNr |
AAH-INF-2-8 |
Targets |
IL6 ;;CCL2;;CCL5;;CCL8;;CXCL10;;ICAM1;;IL15;;IL17A;;TGFB1;;TNFRSF1B |
eClass 6.1 |
32161000 |
eClass 9.0 |
32161000 |
Lieferbar |
|
Short description |
RayBio C-Series Human Inflammation Antibody Array 2 Kit. Detects 20 Human Inflammatory Factors. Suitable for all liquid sample types. |
Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
Number of Targets Detected |
20 |
Target Names |
ICAM-1 (CD54), IL-6 R, IL-15, IL-16, IL-17A, IP-10 (CXCL10), MCP-1 (CCL2), MCP-2 (CCL8), M-CSF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), PDGF-BB |
Targets Detected |
CCL15, CCL2, CCL3, CCL4, CCL5, CCL8, CXCL10, CXCL9, ICAM-1, IL-15, IL-16, IL-17, IL-6 R, IP-10, M-CSF, MCP-1, MCP-2, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PDGF-BB, RANTES, TGF beta 1, TNF alpha, TNF beta, TNF RI, TNF RII, TNFSF1B |
Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process |
Kit Components |
- Human Inflammation Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Membrane |
Design Principle |
Sandwich-based |
Result Output |
Semi-Quantitative |
Detection Method |
Chemiluminescence |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Citation |
Matsunaga K, Yanagisawa S, Ichikawa T, Ueshima K, et al. Airway cytokine expression measured by means of protein array in exhaled breath condensate: Correlation with physiologic properties in asthmatic patients. J Allergy Clin Immunol. 2006, 188:84-90. |
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