Vergleich

Human MMP Array Q1

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Human
Menge 22 Sample Kit
ArtNr QAH-MMP-1-2
Targets MMP2;;MMP3;;MMP9;;TIMP1
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
Quantibody Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs. Suitable for all liquid sample types.
Features
  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 uL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation
Number of Targets Detected
10
Target Names
MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-8, MMP-9, TIMP-1, TIMP-2, TIMP-4
Targets Detected
MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-8, MMP-9, TIMP-1, TIMP-2, TIMP-5
Suggested Applications
Multiplexed Protein Detection, Biomarker Screening, Identifying Key Factors, Confirming Biological Process, Biomarker Validation, Validation of Antibody Array Results, Quantitative Protein Detection, Establishing Normal Range
Kit Components
  • Human MMP Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Chia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 22 Sample Kit
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 25.04.2024 

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