Hersteller |
Raybiotech
|
Kategorie |
|
Typ |
Assay |
Specific against |
Mouse |
Menge |
4 Sample Kit |
ArtNr |
AAM-ANG-G1-4 |
Targets |
IL6 ;; IL9 ;; LEP ;;AXIN1;;CCL11;;CCL2;;FASLG;;FASN;;FOXD3;;IL13;;IL1A;;IL1B;;TACC1;;THPO;;TIMP1 |
eClass 6.1 |
32161000 |
eClass 9.0 |
32161000 |
Lieferbar |
|
Short description |
RayBio G-Series Mouse Angiogenesis Antibody Array 1 Kit. Detects 24 Mouse Angiogenic Factors. Suitable for all liquid sample types. |
Features |
- High sensitivity and specificity
- Low sample volume (10-100 ul per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
|
Number of Targets Detected |
24 |
Target Names |
Eotaxin-1 (CCL11), Fas Ligand (TNFSF6), bFGF, GCSF, GM-CSF, IFN-gamma, IGF-2, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, MCP-1 (CCL2), M-CSF, MIG (CXCL9), Platelet Factor 4 (CXCL4), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), VEGF-A |
Targets Detected |
bFGF, CCL11, CCL2, CXCL14, CXCL4, CXCL9, Eotaxin-1, Fas Ligand, G-CSF, GM-CSF, IFN-gamma, IGF-2, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-12 p40/p70, IL-12 p70, IL-13, IL-6, IL-9, Leptin, M-CSF, MCP-1, MIG, Platelet Factor 4, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNFSF6, TPO |
Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity |
Kit Components |
- Mouse Angiogenesis Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
|
Other Materials Required |
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
|
Protocol Outline |
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
|
Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
Solid Support |
Glass Slide |
Design Principle |
Sandwich-based |
Result Output |
Semi-Quantitative |
Detection Method |
Fluorescence Laser Scanner |
Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Citation |
1. Blansfield J, Caragacianu D, Alexander H, Tangrea M, Morita S, et al. Combining Agents that Target the TumorMicroenvironment Improves the Efficacy of Anticancer Therapy. Clin Cancer Res. 2008, 14(1):270–208. 2. Zhang R, Zhou S-J, Li C-J, et al. C-reactive protein/oxidised low-density lipoprotein/beta2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway. Lipids Health Dis. 2013, 12:42. |
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