|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Assay |
|
Specific against |
Mouse |
|
Menge |
4 Sample Kit |
|
ArtNr |
AAM-ATH-1-4 |
|
eClass 6.1 |
32161000 |
|
eClass 9.0 |
32161000 |
|
Lieferbar |
 |
| Short description |
RayBio C-Series Mouse Atherosclerosis Antibody Array 1 Kit. Detects 22 Mouse Atherosclerotic Factors. Suitable for all liquid sample types. |
| Features |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
|
| Number of Targets Detected |
22 |
| Target Names |
bFGF, CD40 (TNFRSF5), Eotaxin-1 (CCL11), GCSF, GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-13, L-Selectin (CD62L), MCP-1 (CCL2), M-CSF, MIP-3 alpha (CCL20), P-Selectin, RANTES (CCL5), TNF alpha, VEGF-A |
| Targets Detected |
bFGF, CCL11, CCL2, CCL20, CCL5, CD40, CD62L, Eotaxin-1, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-13, IL-2, IL-3, IL-4, IL-5, IL-6, L-Selectin, M-CSF, MCP-1, MIP-3 alpha, P-Selectin, RANTES, TNF alpha, TNFRSF5, VEGF-A |
| Suggested Applications |
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process |
| Kit Components |
- Mouse Atherosclerosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
|
| Other Materials Required |
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
|
| Protocol Outline |
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
|
| Storage |
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months. |
| Solid Support |
Membrane |
| Design Principle |
Sandwich-based |
| Result Output |
Semi-Quantitative |
| Detection Method |
Chemiluminescence |
| Compatible Sample Types |
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
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