Mouse Cytokine Array C1 (8)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Mouse
Menge 8 Sample Kit
ArtNr AAM-CYT-1-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Mouse Cytokine Antibody Array 1 Kit. Detects 22 Mouse Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
22
Target Names
GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17A, IFN-gamma, MCP-1 (CCL2), MCP-5, RANTES (CCL5), SCF, TNF RI (TNFRSF1A), TNF alpha, Thrombopoietin (TPO), VEGF-A
Targets Detected
CCL2, CCL5, G-CSF, GM-CSF, IFN-gamma, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, MCP-1, MCP-5, RANTES, SCF, Thrombopoietin, TNF alpha, TNF RI, TPO
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Wang M, Xiao GG, Li N, Xie Y, Loo JA, Nel AE. Use of a fluorescent phosphoprotein dye to characterize oxidative stress-induced signaling pathway components in macrophage and epithelial cultures exposed to diesel exhaust particle chemicals. Electrophoresis. 2005, 26:2092–2108.
2. Carnevalli L., Scognamiglio R., Cabezas-Wallschied N., Rahmig S., Laurenti E. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B. JEM vol. 211 no. 5 769-779
3. Park J., Li H., Zhang M., Lu Y., Hong B., et al. Murine Th9 cells promote the survival of myeloid dendritic cells in cancer immunotherapy. Cancer Immunology and Immunotherapy, 2014 Epub Ahead of Print. DOI 10.1007/s00262-014-1557-4
4. Resa-Infante P., Thieme R., Ernst T., Arck P., Ittrich H., Reimer R., Gabriel G. Importin-alpha7 is Required for Enhanced Influenza A Virus Replication in the Alveolar Epithelium and Severe Lung Damage in Mice. Journal of Virology Volume 88, Issue 11.
5. Le Quement, C., Guenon, I., Gillon, J.-Y., Valença, S., Cayron-Elizondo, V., Lagente, V. and Boichot, E. (2008), The selective MMP-12 inhibitor, AS111793 reduces airway inflammation in mice exposed to cigarette smoke. British Journal of Pharmacology, 154: 1206–1215. doi: 10.1038/bjp.2008.180
6. Kimura H, Tzou S-C, Rocchi R, Kimura M, et al. Interleukin (IL)-12-Driven Primary Hypothyroidism: the Contrasting Roles of Two Th1 Cytokines (IL-12 and Interferon-gamma). Endocrinol. 2005, 146(8):3642–3651.
7. Kim B., Kim H. Chrysanthemum zawadskii extract activates peroxisome proliferator-activated receptor-alpha. Korean J. Chem. Eng., 31(4), 1-8 (2014). DOI: 10.1007/s11814-014-0109-0 and has an anti-inflammatory activity : Potential interest for the skin barrier function
8. Denis Gallagher, Andreea A. Norman, Cameron L. Woodard, et al. "Transient Maternal IL-6 Mediates Long-Lasting Changes in Neural Stem Cell Pools by Deregulating an Endogenous Self-Renewal Pathway." Cell Stem Cell 13, 564–576, November 7, 2013. http://dx.doi.org/10.1016/j.stem.2013.10.002

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Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 815,26 €
Preis: 815,26 €
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