Mouse Cytokine Array C3 (2)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Mouse
Menge 2 Sample Kit
ArtNr AAM-CYT-3-2
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Mouse Cytokine Antibody Array 3 Kit. Detects 62 Mouse Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
62
Target Names
Axl, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Fas Ligand (TNFSF6), Fractalkine (CX3CL1), GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17A, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-9, KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), Platelet Factor 4 (CXCL4), P-Selectin, RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TIMP-1, TNF alpha, Thrombopoietin (TPO), VCAM-1 (CD106), VEGF-A
Targets Detected
AXL, BLC, CCL1, CCL11, CCL17, CCL19, CCL2, CCL20, CCL24, CCL25, CCL27, CCL3, CCL5, CD106, CD30, CD30 Ligand, CD40, CD62L, CRG-2, CTACK, CX3CL1, CXCL1, CXCL12 alpha, CXCL13, CXCL14, CXCL16, CXCL4, CXCL9, Eotaxin-1, Eotaxin-2, Fas Ligand, Fractalkine, G-CSF, GM-CSF, I-309, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-9, KC, L-Selectin, Leptin, Leptin R, LIX, Lymphotactin, M-CSF, MCP-1, MCP-5, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MPIF-2, P-Selectin, Platelet Factor 4, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, Thrombopoietin, TIMP-1, TNF alpha, TNFRSF5, TNFRSF8, TNFSF6, TNFSF8, TPO, VCAM-1, VEGF-A, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Mouse Cytokine Antibody Array C3 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Zhou Q, Desta T, Fenton M, Graves DT, Amar S. Cytokine Profiling of Macrophages Exposed to Porphyromonas gingivalis, Its Lipopolysaccharide, or Its FimA Protein. Infect Immun. 2005, 73(2):935–943.
2. Orientia tsutsugamushi subverts dendritic cell functions by escaping from autophagy and impairing their migration
3. Cyclophilin D Extramitochondrial Signaling Controls Cell Cycle Progression and Chemokine-Directed Cell Motility
4. Kang TW., et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature. 2011 Nov 9, 479(7374):547-51. doi: 10.1038/nature10599.
5. Kang TW., et al. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature. 2011 Nov 9, 479(7374):547-51. doi: 10.1038/nature10599.
6. Li, H et al. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. IL-1 receptor-associated kinase M is a central regulator of osteoclast differentiation and activation. J. Exp. Med. Volume 201, Issue 7, Pages 1169-77, Apr 2005
7. Acres B, Gantzer M, Remy C, Futin N, Accart N, et al. Fusokine Interleuikin-2/Interleukin-18, a Novel Potent Innate and Adaptive Immune Stimulator with Decreased Toxicity. Cancer Res. 2005, 65(20):9536–9546.
8. Venmar K., Carter K., Hwang D., Dozier A., Fingleton B. IL-4 receptor ILR4alpha regulates metastatic colonization by mammary tumors through multiple signaling pathways. Cancer Research Published OnlineFirst June 19, 2014, doi: 10.1158/0008-5472.CAN-14-0093
9. Ehninger D. Tsc2 Haploinsufficiency Has Limited Effects on Fetal Brain Cytokine Levels during Gestational Immune Activation. Autism Research and Treatment. Volume 2014, Article ID 761279, 7 pages http://dx.doi.org/10.1155/2014/761279
10. Price P., et al. Chemokine (C-C motif) receptor 1 (CCR1) is required for efficient recruitment of neutrophils during respiratory infection with Modified Vaccinia virus Ankara J. Virol. published ahead of print 9 July 2014 , doi:10.1128/JVI.01524-14
11. Vilatla M., et al. Recruitment of Circulating Breast Cancer Cells Is Stimulated by Radiotherapy. Cell Available online 10 July 2014. http://dx.doi.org/10.1016/j.celrep.2014.06.011
12. Palmi C., et al. Cytoskeletal Regulatory Gene Expression and Migratory Properties of B Cell Progenitors are Affected by the ETV6-RUNX1 Rearrangement Mol Cancer Res molcanres.0056.2014, Published OnlineFirst July 24, 2014, doi: 10.1158/1541-7786.MCR-14-0056-T
13. Ha CT, Li X-H, Fu D, Moroni M, Fisher C, et al. (2014) Circulating Interleukin-18 as a Biomarker of Total-Body Radiation Exposure in Mice, Minipigs, and Nonhuman Primates (NHP). PLoS ONE 9(10): e109249. doi:10.1371/journal.pone.0109249
14. Llorens-Martin M., et al. Peripherally triggered and GSK-3beta-driven brain inflammation differentially skew adult hippocampal neurogenesis, behavioral pattern separation and microglial activation in response to ibuprofen. Translational Psychiatry (2014) 4, e463, doi:10.1038/tp.2014.92, published online 14 October 2014
15. Benabid R., et al. Neutrophil Elastase Modulates Cytokine Expression: CONTRIBUTION TO HOST DEFENSE AGAINST PSEUDOMONAS AERUGINOSA-INDUCED PNEUMONIA. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 287, NO. 42, pp. 34883–34894, October 12, 2012, DOI 10.1074/jbc.M112.361352

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Das dargestellte Dokument entspricht unserem letzten Update und sollte lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 2 Sample Kit
Lieferbar: In stock
Listenpreis: 472,99 €
Preis: 472,99 €
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