Mouse Cytokine Array G1000 (8)

Hersteller Raybiotech
Kategorie
Typ Assay
Specific against Mouse
Menge 8 Sample Kit
ArtNr AAM-CYT-G1000-8
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Mouse Cytokine Antibody Array 1000. A combination of Mouse Cytokine Antibody Array G3 & G4. Detects 96 Mouse Cytokines. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
96
Target Names
Axl, bFGF, BLC (CXCL13), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD40 (TNFRSF5), CRG-2, CTACK (CCL27), CXCL16, CD26 (DPPIV), Dtk, Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), GCSF, GITR (TNFRSF18), GM-CSF, HGFR, ICAM-1 (CD54), IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17 RB, IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, I-TAC (CXCL11), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MCP-1 (CCL2), MCP-5, M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Platelet Factor 4 (CXCL4), Pro-MMP-9, P-Selectin, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TROY (TNFRSF19), TSLP, VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D
Targets Detected
AXL, bFGF, BLC, CCL1, CCL11, CCL17, CCL19, CCL2, CCL20, CCL22, CCL24, CCL25, CCL27, CCL3, CCL5, CD106, CD26, CD30, CD30 Ligand, CD32b, CD40, CD54, CD62L, CRG-2, CTACK, CX3CL1, CXCL1, CXCL11, CXCL12 alpha, CXCL13, CXCL14, CXCL15, CXCL16, CXCL4, CXCL7, CXCL9, DPPIV, Dtk, E-Selectin, Eotaxin-1, Eotaxin-2, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, G-CSF, GITR, GM-CSF, HGFR, I-309, I-TAC, ICAM-1, IFN-gamma, IGF-1, IGF-2, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17, IL-17B R, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-7, IL-9, KC, L-Selectin, Leptin, Leptin R, LIX, Lungkine, Lymphotactin, M-CSF, MCP-1, MCP-5, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, MMP-3, MPIF-2, Osteopontin, Osteoprotegerin, P-Selectin, Platelet Factor 4, Pro-MMP-9, RANTES, Resistin, SCF, SDF-1 alpha, Shh-N, Sonic Hedgehog N-Terminal, SPP1, TARC, TCA-3, TCK-1, TECK, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNFRSF11B, TNFRSF18, TNFRSF19, TNFRSF5, TNFRSF8, TNFSF11, TNFSF6, TNFSF8, TPO, TRANCE, TROY, TSLP, VCAM-1, VEGF-A, VEGF-D, VEGFR1, VEGFR2, VEGFR3, XCL1
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Mouse Cytokine Antibody Array G1000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Litvinov D., et al. Anti-atherosclerotic actions of azelaic acid, an end product of linoleic acid peroxidation, in mice. Atherosclerosis. 2010 Apr, 209(2):449-54. doi: 10.1016/j.atherosclerosis.2009.09.076.
2. Labelle M., et al. Platelets guide the formation of early metastatic niches. PNAS published online July 2014. www.pnas.org/cgi/doi/10.1073/pnas.1411082111
Menge: 8 Sample Kit
Lieferbar: In stock
Listenpreis: 2.319,01 €
Preis: 2.319,01 €
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