Mouse Inflammation Array C1

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

BLC (CXCL13), CD30 Ligand (TNFSF8), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Fas Ligand (TNFSF6), Fractalkine (CX3CL1), GCSF, GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17A, I-TAC (CXCL11), KC (CXCL1), Leptin, LIX, Lymphotactin (XCL1), MCP-1 (CCL2), M-CSF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, RANTES (CCL5), SDF-1 alpha (CXCL12 alpha), I-309 (TCA-3/CCL1), TECK (CCL25), TIMP-1, TIMP-2, TNF alpha, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B)

Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.

Sandwich-based

Chemiluminescence

1. Wu Z., Chen X., Liu F., Chen W., Wu P., Wieschhaus A., Chishti A., Roche P., Chen W., Lin TJ. Calpain-1 Contributes to IgE-Mediated Mast Cell Activation. The Journal of Immunology April 23, 2014 1301677. doi: 10.4049/? [Epub ahead of print]
2. Ke, F., Zhang, L. et al. Autocrine IL-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca2+ channels. Stem Cells 2014. Epub Ahead of Print. DOI: 10.1002/stem.1763
3. Soki F., Polarization of Prostate Cancer Associated Macrophages is Induced by Milk-Fat Globule-EGF Factor 8 (MFG-E8) Mediated Efferocytosis. JBC Papers in Press. Published on July 8, 2014. http://www.jbc.org/cgi/doi/10.1074/jbc.M114.571620
4. Sun H., Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection. International Immunopharmacology 22 (2014) 346–355. http://dx.doi.org/10.1016/j.intimp.2014.07.021
5. Adkins I., et al. Bordetella Adenylate Cyclase Toxin Differentially Modulates Toll-Like Receptor-Stimulated Activation, Migration and T Cell Stimulatory Capacity of Dendritic Cells. PLOS One 2014. Published: August 01, 2014. DOI: 10.1371/journal.pone.0104064
6. Tsuyusaki J., et al. Cigarette smoke-induced pulmonary inflammation is attenuated in CD69-deficient mice. Journal of Receptors and Signal Transduction, 2011, 31(6): 434–439, DOI: 10.3109/10799893.2011.631929
7. Lesina E., et al. CpG-free plasmid DNA prevents deterioration of pulmonary function in mice. European Journal of Pharmaceutics and Biopharmaceutics 74 (2010) 427–434. doi:10.1016/j.ejpb.2009.11.013