Rat Cytokine Array C1

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

CINC-2, CINC-3, CNTF, Fractalkine (CX3CL1), GM-CSF, IFN-gamma, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-4, IL-6, IL-10, LIX, Leptin, MCP-1 (CCL2), MIP-3 alpha (CCL20), beta-NGF, TIMP-1, TNF alpha, VEGF-A

Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process

• Pipettors, pipet tips and other common lab consumables
• Orbital shaker or oscillating rocker
• Tissue Paper, blotting paper or chromatography paper
• Adhesive tape or Saran Wrap
• Distilled or de-ionized water
• A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.

Sandwich-based

Chemiluminescence

1. Deuse T., Hua X., Wang D., Maegdefessel L., et al. Dichloroacetate prevents restenosis in preclinical animal models of vessel injury. Nature (2014) doi:10.1038/nature13232 [Epub ahead of print]
2. Schmelzer K, Wheelock Å, Dettmer K , Morin D, Hammock B.The role of Inflammatory Mediators in the Synergistic Toxicity of Ozone and 1-Nitronaphthalene in Rat Airways.Environ Health Perspect. 2006 , 114(9): 1354–1360.
3. Liao, H et al. Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function. Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function. Tenascin-R plays a role in neuroprotection via its distinct domains that coordinate to modulate the microglia function. J. Biol. Chem. Volume 280, Issue 9, Pages 8316-23, Mar 2005
4. Moreau, R et al. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Reversal by aminoguanidine of the age-related increase in glycoxidation and lipoxidation in the cardiovascular system of Fischer 344 rats. Biochem. Pharmacol. Volume 69, Issue 1, Pages 29-40, Jan 2005