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Mouse Apoptosis Signaling Pathway Array C1

Mouse Apoptosis Signaling Pathway Array C1

ArtNr	AAM-APOSIG-1-4
Hersteller	Raybiotech
Menge	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit 8 Sample Kit
Kategorie	Cardiovascular Research Ischemia & Reperfusion Injury Arrays Antibody Arrays
Typ	Array
Specific against	Mouse (Murine, Mus musculus)
Host	Hamster - Armenian
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Manufacturer - Category	Arrays\|Post-Translational Modification Arrays\|Phosphorylation Arrays\|Apoptosis Arrays\|Antibody Arrays\|Membrane Antibody Arrays
Manufacturer - Targets	AKT, bad, ERK1/2, p53, Caspase-3, HSP27, ATM, Caspase-7, CHK1, eIF-2a, IkB-alpha, JNK, NF-kB P65, SMAD2, TAK1, p38, p27/Kip1 / CDKN1B
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Research Area	Apoptosis, Akt Signaling, HER/ErbB Signaling, HIF-1 alpha Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, TGF-beta Signaling, p53 Signaling, NFkB Signaling, PKC Signaling
Description	C-Series Mouse Apoptosis Antibody Array 1 Kit. Detects 17 phosphorylated or cleaved mouse Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Number of Targets Detected	17
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	AKT1 \| ATM \| BAD \| CASP3 \| CASP7 \| CDKN1B \| CHEK1 \| EIF2S1 \| HSPB1 \| MAP3K7\| MAPK14 \| MAPK8 \| NFKBIA \| RELA \| SMAD2 \| TP53
Kit Components	Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes Blocking Buffer Detection Antibody Cocktail1, 000 X HRP-Anti-Rabbit IgG Concentrate20 X Wash Buffer I Concentrate20 X Wash Buffer I I Concentrate2 X Cell Lysis Buffer Concentrate Detection Buffer C Detection Buffer D8-Well Incubation Tray w/ Lid Protease Inhibitor Cocktail100x Phosphatase Inhibitor Cocktail I Phosphatase Inhibitor Cocktail IIPlastic Sheets Array Map Template Manual
Other Materials Required	Pipettors, pipet tips and other common lab consumables Orbital shaker or oscillating rocker Tissue Paper, blotting paper or chromatography paper Adhesive tape or Saran Wrap Distilled or de-ionized water A chemiluminescent blot documentation system (such as UVP's Chemi Doc-It® or Epi Chem I I Benchtop Darkroom or G E's Image Quant„¢ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Block membranes Incubate with Sample Incubate with Detection Antibody Cocktail Incubate with HRP-Conjugated anti-IgGIncubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
UNSPSC Code	41116133
Pathway	Akt SignalingHER/ErbB SignalingHIF-1 alpha SignalingIGF SignalingJAK/STAT SignalingMAPK SignalingmTOR SignalingNFkB Signalingp53 SignalingPI3K-AKT SignalingPKC SignalingTGF-beta Signaling

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