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Human c-Fos Activity Assay Kit

ArtNr TFEH-CFOS-5
Hersteller Raybiotech
Menge 5 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens)
Host Hamster - Armenian
Dry ice Yes
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Versandbedingung Trockeneis
Lieferbar
Specificity The olionucleotide/antibody pair provided in this kit recognizes human c-Fos in whole lysates and nuclear extracts.
Manufacturer - Category
ELISA Kits|Transcription Factor Activity Assays
Shipping Temperature
Dry ice (additional shipping fee)
Storage Conditions
-20°C
Compatible Sample Types
Cell Lysates
Nuclear Extracts
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
96-well Microplate
Gene Symbols
FOSG0S7
Kit Components
96-well Strip Microplate pre-coated with DNA probesDN
A Binding Buffer
Positive Control Sample
Specific Competitor DNA probe
Non-specific Competitor DNA probe
Assay ReagentDTTWash Buffer
Primary Antibody
HRP-conjugated Secondary Antibody
Antibody Diluent Buffer
TMB One-Step Substrate Reagent
Stop Solution
Other Materials Required
Distilled or deionized water 100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Absorbent paper
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
Prepare all reagents and samples as instructed in the manual.
Add 100 µl of sample or positive control to each well.
Incubate 2 h at RT or O/N at 4°C.
Add 100 µl of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared HRP-secondary antibody to each well.
Incubate 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Protein Name & Synonyms
Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7
UNSPSC Code
41116158
Manufacturer - Specificity
The olionucleotide/antibody pair provided in this kit recognizes human c-Fos in whole lysates and nuclear extracts.
Pathway
IGF SignalingJAK/STAT SignalingMAPK SignalingPI3K-AKT SignalingPKC SignalingTGF-beta Signaling

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5 Plate Kit
Lieferbar: In stock
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