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Human IL-1 beta ELISA Kit

Human IL-1 beta ELISA Kit

ArtNr	ELH-IL1b-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Cholesterol & Lipid Metabolism Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Bone Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	0.3 pg/ml
Citations	1. Ertugrul AS, Sahin H, Dikilitas A, et al. Comparison of CCL28, interleukin-8, interleukin-1beta and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis. J Periodont Res. 2013 Feb,48(1):44–51. 2. Verma P, Dalal K. Serum cartilage oligomeric matrix protein (COMP) in knee osteoarthritis: A novel diagnostic and prognostic biomarker. J Orthop Res. 2013 Jul,31(7):999–1006. 3. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536 4. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-beta, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62 5. Chowdhurya I.H., et al. Alteration of serum inflammatory cytokines in active pulmonarytuberculosis following anti-tuberculosis drug therapy. Molecular Immunology 62 (2014) 159–168 6. Abdallah M., et al. Serum Th1/Th2 and Macrophage Lineage Cytokines in Leprosy, Correlation with Circulating CD4+ CD25highFoxP3+ T-regs Cells. Experimental Dermatology, August 2014 Accepted Article, doi:10.1111/exd.12529 7. Scherer O, Steinmetz H, Kaether C, Weinigel C, Barz D, Kleinert H, Menche D, M¨uller R, Pergola C, Werz O, Targeting V-ATPase in primary human monocytes by archazolid potently represses the classical secretion of cytokines due to accumulation at the endoplasmic reticulum, Biochemical Pharmacology (2014), http://dx.doi.org/10.1016/j.bcp.2014.07.028 8. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111. 9. Bishayi B., et al. Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes. Inflammation August 2014, Epub ahead of print. DOI: 10.1007/s10753-014-9991-1 10. Leyh et al.: Subchondral bone influences chondrogenic differentiation and collagen production of human bone marrow-derived mesenchymal stem cells and articular chondrocytes. Arthritis Research & Therapy 2014 16:453. doi:10.1186/s13075-014-0453-9
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	IL-1 beta, IL-1 F2
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	IL-1 beta / IL-1F2
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Human IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IL1B, IL1F2
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Interleukin-1 beta (IL-1 beta) (Catabolin)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Recommended Dilution	2 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELH-IL1b-5.pdf

ELH-IL1b-5-datasheet.pdf

ELH-IL1b-5-safety-datasheet.pdf