Human IL-6 ELISA

Hersteller Raybiotech
Kategorie
Typ Elisa-Kit
Specific against Human
Menge 1 x 96-Well Strip Microplate Kit
Host E.coli
ArtNr ELH-IL6-1
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Quantity options
Short description
RayBio Human IL-6 ELISA Kit for serum, plasma, cell culture supernatants, and urine
Target Name and Synonyms
IL-6
Solid Support
96-well Microplate
Design Principle
Sandwich-based
Result Output
Quantitative
Method of Detection
Colorimetric
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Urine
Sensitivity
3 pg/ml
Detection Range
3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF).
Product Features
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Summary
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Storage
May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
Citation
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4. Cell-line-specific stimulation of tumor cell aggressiveness by wound healing factors -- a central role for STAT3
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8. Deangelo DJ, Mesa RA, Fiskus W, et al. Phase II trial of panobinostat, an oral pan-deacetylase inhibitor in patients with primary myelofibrosis, post-essential thrombocythaemia, and post-polycythaemia vera myelofibrosis. Br J Haematol. 2013 Aug, 162(3):326–335.
9. Bassyouni IH, El-Wakd MM, Bassyouni RH. Soluble levels of osteopontin in patients with Behcet’s disease: association with disease activity and vascular involvement. J Clin Immunol. 2013 Feb, 33(2):361–367.
10. Gaber W, Azkalany GS, Gheita TA, et al. Clinical significance of serum interleukin-6 and -174 G/C promoter polymorphism in Rheumatoid arthritis patients. Egypt Rheumatol. 2013 Apr, 35(2):107–113.
11. Rocher, Crystal, and Dinender K. Singla. "SMAD-PI3K-Akt-mTOR Pathway Mediates BMP-7 Polarization of Monocytes into M2 Macrophages." PLoS One. NCBI, 20 Dec. 2013. Web.
12. Bassyouni I., El-Wakd M., Azab N., Bassyouni RH. Diminished Soluble levels of growth arrest specific protein 6 and tyrosine kinase receptor Axl in patients with rheumatoid arthritis. International Journal of Rheumatic Diseases, 7 APR 2014 [Epub Ahead of Print]
13. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
14. Guglielmetti S, Tamagnini I, Mora D, Minuzzo M , Scarafoni A, et al.Implication of an Outer Surface Lipoprotein in Adhesion of Bifidobacterium bifidum to Caco-2 Cells. Appl Environ Microbiol. 2008:74(15):4695–4702.
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16. Abed chabuk S., Al-Momen A., Alsamaraee I. Correlation Between Interleukins 1 alpha, 6 and Echocardiographic Results in Patients with Heart Failure. Journal of Babylon University/Pure and Applied Sciences/ No.(4)/ Vol.(22): 2014
17. Hung P., Tsai H., Lin C., Hung K. Abdominal Obesity is Associated with Peripheral Artery Disease in Hemodialysis Patients. PLOS One Published: August 02, 2013 DOI: 10.1371/journal.pone.0069424
18. Xie, J et al. Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. J. Virol. Volume 79, Issue 24, Pages 15027-37, Dec 2005
19. Rattray B., Steel J., Semple S., Ramirez R., Abhayaratna WP. Short-term eccentric exercise in newly diagnosed type II diabetics: an exploratory study. Sport Sciences for Health, June 2014 Epub ahead of print
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21. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
22. Sharma M., et al. In Vitro Anticancer Activity of Plant-Derived Cannabidiol on Prostate Cancer Cell Lines
23. Chowdhurya I.H., et al. Alteration of serum inflammatory cytokines in active pulmonarytuberculosis following anti-tuberculosis drug therapy. Molecular Immunology 62 (2014) 159–168
24. Lakshmi J., et al. Economical Production of Recombinant Human Interleukin–17E using Industrially Important Salt Inducible Escherichia coli GJ1158: A Fermentation Approach. International Journal of Advanced Science and Technology Vol.68 (2014), pp.23-36. http://dx.doi.org/10.14257/ijast.2014.68.03
25. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
26. Bishayi B., et al. Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes. Inflammation August 2014, Epub ahead of print. DOI: 10.1007/s10753-014-9991-1
27. Elsaid A., et al. Association of polymorphisms G(-174)C in IL-6 gene and G(-1082)A in IL-10 gene with tranditional cardiovascular risk factors in patients with coronary artery disease. Indian Journal of Biochemistry and Biophysics. Vol 51, August 2014, pp 282-292
28. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
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30. Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041
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Arbeitsanleitung

ELH-IL6-1.pdf

Menge: 1 x 96-Well Strip Microplate Kit
Lieferbar: In stock
Listenpreis: 482,90 €
Preis: 482,90 €
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