Human IL-6 ELISA

RayBio Human IL-6 ELISA Kit for serum, plasma, cell culture supernatants, and urine

96-well Microplate

Quantitative

Cell Culture Supernatants, Plasma, Serum, Urine

3 pg/ml - 1000 pg/ml

This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF).

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 ul of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4C.
4. Add 100 ul of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 ul of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.

1. Said NA, Najwer I, Socha MJ, Fulton DJ, Mok SC, Motamed K. SPARC Inhibits LPA-Mediated Mesothelial-Ovarian Cancer Cell Crosstalk. Neoplasia. 2007, 9(1):23–35.
2. Involvement of corneal epithelial cells in the Th17 response in an in vitro bacterial inflammation model
3. Oh E-T, Park M-T, Song M-J, et al. Radiation-induced angiogenic signaling pathway in endothelial cells obtained from normal and cancer tissue of human breast. Oncogene. 2013 Mar 18, [Epub ahead of print] doi:10.1038/onc.2013.70.
4. Cell-line-specific stimulation of tumor cell aggressiveness by wound healing factors -- a central role for STAT3
5. El-Awady AR, Lapp CA, Gamal AY, et al. Human periodontal ligament fibroblast responses to compression in chronic periodontitis. J. Clin. Periodontol. 2013 Jul, 40(7):661–671.
6. Wu SJ, Lu TM, Lai MN, Ng LT. Immunomodulatory Activities of Medicinal Mushroom Grifola frondosa Extract and Its Bioactive Constituent. Am J Chin Med. 2013, 41:131. doi: 10.1142/S0192415X13500109
7. Stockert J, Wolf A, Kaddatz K, et al. Regulation of TAK1/TAB1-mediated IL-1beta signaling by cytoplasmic PPARbeta/delta. PLoS ONE. 2013, 8(4):e63011.
8. Deangelo DJ, Mesa RA, Fiskus W, et al. Phase II trial of panobinostat, an oral pan-deacetylase inhibitor in patients with primary myelofibrosis, post-essential thrombocythaemia, and post-polycythaemia vera myelofibrosis. Br J Haematol. 2013 Aug, 162(3):326–335.
9. Bassyouni IH, El-Wakd MM, Bassyouni RH. Soluble levels of osteopontin in patients with Behcet’s disease: association with disease activity and vascular involvement. J Clin Immunol. 2013 Feb, 33(2):361–367.
10. Gaber W, Azkalany GS, Gheita TA, et al. Clinical significance of serum interleukin-6 and -174 G/C promoter polymorphism in Rheumatoid arthritis patients. Egypt Rheumatol. 2013 Apr, 35(2):107–113.
11. Rocher, Crystal, and Dinender K. Singla. "SMAD-PI3K-Akt-mTOR Pathway Mediates BMP-7 Polarization of Monocytes into M2 Macrophages." PLoS One. NCBI, 20 Dec. 2013. Web.
12. Bassyouni I., El-Wakd M., Azab N., Bassyouni RH. Diminished Soluble levels of growth arrest specific protein 6 and tyrosine kinase receptor Axl in patients with rheumatoid arthritis. International Journal of Rheumatic Diseases, 7 APR 2014 [Epub Ahead of Print]
13. Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
14. Guglielmetti S, Tamagnini I, Mora D, Minuzzo M , Scarafoni A, et al.Implication of an Outer Surface Lipoprotein in Adhesion of Bifidobacterium bifidum to Caco-2 Cells. Appl Environ Microbiol. 2008:74(15):4695–4702.
15.
16. Abed chabuk S., Al-Momen A., Alsamaraee I. Correlation Between Interleukins 1 alpha, 6 and Echocardiographic Results in Patients with Heart Failure. Journal of Babylon University/Pure and Applied Sciences/ No.(4)/ Vol.(22): 2014
17. Hung P., Tsai H., Lin C., Hung K. Abdominal Obesity is Associated with Peripheral Artery Disease in Hemodialysis Patients. PLOS One Published: August 02, 2013 DOI: 10.1371/journal.pone.0069424
18. Xie, J et al. Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. Kaposi's sarcoma-associated herpesvirus induction of AP-1 and interleukin 6 during primary infection mediated by multiple mitogen-activated protein kinase pathways. J. Virol. Volume 79, Issue 24, Pages 15027-37, Dec 2005
19. Rattray B., Steel J., Semple S., Ramirez R., Abhayaratna WP. Short-term eccentric exercise in newly diagnosed type II diabetics: an exploratory study. Sport Sciences for Health, June 2014 Epub ahead of print
20. Lakshmi J., Kotra S., Peravali J., Kumar P
21. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
22. Sharma M., et al. In Vitro Anticancer Activity of Plant-Derived Cannabidiol on Prostate Cancer Cell Lines
23. Chowdhurya I.H., et al. Alteration of serum inflammatory cytokines in active pulmonarytuberculosis following anti-tuberculosis drug therapy. Molecular Immunology 62 (2014) 159–168
24. Lakshmi J., et al. Economical Production of Recombinant Human Interleukin–17E using Industrially Important Salt Inducible Escherichia coli GJ1158: A Fermentation Approach. International Journal of Advanced Science and Technology Vol.68 (2014), pp.23-36. http://dx.doi.org/10.14257/ijast.2014.68.03
25. Gotoh M., et al. Cyclic phosphatidic acid relieves osteoarthritis symptoms. Gotoh et al. Molecular Pain 2014, 10:52. doi:10.1186/1744-8069-10-52
26. Bishayi B., et al. Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes. Inflammation August 2014, Epub ahead of print. DOI: 10.1007/s10753-014-9991-1
27. Elsaid A., et al. Association of polymorphisms G(-174)C in IL-6 gene and G(-1082)A in IL-10 gene with tranditional cardiovascular risk factors in patients with coronary artery disease. Indian Journal of Biochemistry and Biophysics. Vol 51, August 2014, pp 282-292
28. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
29.
30. Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041
31. Chen Z., et al. IL-6, IL-10 and IL-13 are associated with pathogenesis in children with Enterovirus 71 infection. Int J Clin Exp Med 2014, 7(9):2718-2723