« Zurück
Startseite /
Human TNF alpha ELISA Kit

Human TNF alpha ELISA Kit

ArtNr	ELH-TNFa-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes By Cell Type Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Breast Cancer Renal Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	30 pg/ml
Citations	1. Rocher, Crystal, and Dinender K. Singla. "SMAD-PI3K-Akt-mTOR Pathway Mediates BMP-7 Polarization of Monocytes into M2 Macrophages." PLoS One. NCBI, 20 Dec. 2013. Web. 2. Omoomi FD. Molecular Chlorambucil-Methionine Conjugate: Novel Anti-cancer Agent against Breast MCF-7 Cell Model. J Cancer Sci Therapy. 2013,05(02). 3. Wu SJ, Lu TM, Lai MN, Ng LT. Immunomodulatory Activities of Medicinal Mushroom Grifola frondosa Extract and Its Bioactive Constituent. Am J Chin Med. 2013,41:131. doi: 10.1142/S0192415X13500109 4. Leow K-Y, Goh WWB, Tan S-Z, et al. Variant screening of the serum amyloid A1 gene and functional study of the p.Gly90Asp variant for its role in atherosclerosis. Atherosclerosis. 2013 Mar,227(1):112–117. 5. Ertugrul AS, Sahin H, Dikilitas A, et al. Comparison of CCL28, interleukin-8, interleukin-1beta and tumor necrosis factor-alpha in subjects with gingivitis, chronic periodontitis and generalized aggressive periodontitis. J Periodont Res. 2013 Feb,48(1):44–51. 6. Deangelo DJ, Mesa RA, Fiskus W, et al. Phase II trial of panobinostat, an oral pan-deacetylase inhibitor in patients with primary myelofibrosis, post-essential thrombocythaemia, and post-polycythaemia vera myelofibrosis. Br J Haematol. 2013 Aug,162(3):326–335. 7. Verma P, Dalal K. Serum cartilage oligomeric matrix protein (COMP) in knee osteoarthritis: A novel diagnostic and prognostic biomarker. J Orthop Res. 2013 Jul,31(7):999–1006. 8. Llamas-Covarrubias MA, Valle Y, Bucala R, et al. Macrophage migration inhibitory factor (MIF): Genetic evidence for participation in early onset and early stage rheumatoid arthritis. Cytokine. 2013,61(3):759–765. 9. Fukumoto J, Fukumoto I, Parthasarathy PT, et al. NLRP3 Deletion Protects from Hyperoxia-Induced Acute Lung Injury. Am J Physiol Cell Physiol. 2013 Jul,305(2):C182–C189. 10. Chhabra G.S., Kulkarni K.S. Evaluation of anti-inflammatory activity of Vitex agnus castus leaves. Quantitative analysis of flavonoids as possible active constituents. ca. 183 ca. Journal of Pharmacognosy and Phytochemistry 2014, 3 (1): 183-189 11. Hung P., Tsai H., Lin C., Hung K. Abdominal Obesity is Associated with Peripheral Artery Disease in Hemodialysis Patients. PLOS One Published: August 02, 2013 DOI: 10.1371/journal.pone.0069424 12. Rattray B., Steel J., Semple S., Ramirez R., Abhayaratna WP. Short-term eccentric exercise in newly diagnosed type II diabetics: an exploratory study. Sport Sciences for Health, June 2014 Epub ahead of print 13. Bhaktha G., et al. SERUM TNF-alpha AND LEPTIN LEVELS IN GRADED OBESE DIABETIC WOMEN- FREE FROM MICRO AND MACRO VASCULAR COMPLICATIONS. IJPCBS 2014, 4(3), 648-653 14. Bishayi B., et al. Expression of CXCR1 (Interleukin-8 Receptor) in Murine Macrophages After Staphylococcus aureus Infection and its Possible Implication on Intracellular Survival Correlating with Cytokines and Bacterial Anti-Oxidant Enzymes. Inflammation August 2014, Epub ahead of print. DOI: 10.1007/s10753-014-9991-1 15. Shaker MJ., et al. Immunological Study of Women Infected with Trichomonas vaginalis Parasite in Baghdad city. International Journal of Science and Research. Volume 3 Issue 9, September 2014, 16. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 – 1883
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	TNF alpha
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	TNF alpha
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Human TNF alpha ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. 30 pg/ml sensitivity.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	TNF, TNFA, TNFSF2
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4
Recommended Dilution	2 fold

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELH-TNFa-5.pdf

ELH-TNFa-5-datasheet.pdf

ELH-TNFa-5-safety-datasheet.pdf