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Human VCAM-1 ELISA Kit

Human VCAM-1 ELISA Kit

ArtNr	ELH-VCAM1-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neuroinflammation Cell Biology Cell Movement Cell Adhesion ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Renal Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	300 pg/ml
Citations	1. Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes 2. Fadel F., Elshamaa M., Nabhan M., Essam R., Kantoush N., et al. Soluble adhesion molecules as markers of native arteriovenous fistula thrombosis in children on uremia. Blood Coagulation and Firbrinolysis. Published online DOI:10.1097/MBC.0000000000000125 [Epub ahead of Print] 3. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-beta, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	VCAM-1, CD106
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	VCAM-1 / CD106
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Human VCAM-1 (CD106) ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	L1CAM, VCAM1
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Vascular cell adhesion protein 1 (V-CAM 1) (VCAM-1) (INCAM-100) (CD antigen CD106)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4, DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, MCSF R, MMP-1, 2, 3, 7, 8, 9, 10, 12 and 13, PDGF-AB, SDF-1b, Tie-1, Tie- 2, TIMP-3.
Recommended Dilution	10 - 50 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELH-VCAM1-2.pdf

ELH-VCAM1-2-datasheet.pdf

ELH-VCAM1-2-safety-datasheet.pdf