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Human VEGF-A ELISA

Human VEGF-A ELISA

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Hersteller	Raybiotech
Kategorie	ELISA Oncology Bladder Cancer Testicular & Prostate Cancer Lung Cancer Breast Cancer Heart & Blood Cancer Lymphatic System Cancer Uterine, Ovarian & Cervical Cancer Gastric Cancer Pancreatic Cancer Brain Cancer Renal Cancer Nervous System Cancer Head and Neck Cancers
Typ	Elisa-Kit
Specific against	Human
Menge	1 x 96-Well Strip Microplate Kit
Host	E.coli
ArtNr	ELH-VEGF-1
eClass 6.1	32160605
eClass 9.0	32160605
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Short description	RayBio Human VEGF-A ELISA Kit for serum, plasma, cell culture supernatants, and urine
Target Name and Synonyms	VEGF-A
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Result Output	Quantitative
Method of Detection	Colorimetric
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Urine
Sensitivity	10 pg/ml
Detection Range	10 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma)	2-5 fold
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 beta, MIP-1gamma, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO).
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Summary	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Storage	May be stored for up to 6 months at 2C to 8C from the date of shipment. Standard (recombinant protein) should be stored at -20C or -80C (recommended at -80C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2C to 8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20C.
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