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Human VEGF R2 ELISA Kit

Human VEGF R2 ELISA Kit

ArtNr	ELH-VEGFR2-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Hepatic Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	70 pg/ml
Citations	1. Navid F, Baker SD, McCarville B, et al. Phase I and Clinical Pharmacology Study of Bevacizumab, Sorafenib, and Low-Dose Cyclophosphamide in Children and Young Adults with Refractory/Recurrent Solid Tumors. Clin Cancer Res. 2013,19(1):236–246. 2. Zheng YB., et al. The relationship of kinase insert domain receptor gene polymorphisms and clinical outcome in advanced hepatocellular carcinoma patients treated with sorafenib. Med Oncol (2014) 31:209. DOI 10.1007/s12032-014-0209-z
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	VEGFR2
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	VEGFR2
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Human VEGFR2 ELISA Kit for cell culture supernatants, plasma, and serum samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	KDR, FLK1, VEGFR2
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Vascular endothelial growth factor receptor 2 (VEGFR-2) (EC 2.7.10.1) (Fetal liver kinase 1) (FLK-1) (Kinase insert domain receptor) (KDR) (Protein-tyrosine kinase receptor flk-1) (CD antigen CD309)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiopoietin-1, Angiostatin , BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, ESelectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Recommended Dilution	10 - 100 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELH-VEGFR2-2.pdf

ELH-VEGFR2-2-datasheet.pdf

ELH-VEGFR2-2-safety-datasheet.pdf