Vergleich

Human/Mouse/Rat Phospho-Stat 3 (Y705) Cell-Based ELISA

Hersteller Raybiotech
Kategorie
Typ Elisa-Kit
Specific against Human, Mouse, Rat
Menge 5 Plate Kit
Host Hamster - Armenian
ArtNr CBEL-STAT3-5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Species Detected
Human Mouse Rat
Compatible Sample Types
Adherent Cell Culture
Design Principle
Cell-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
96-well Microplate
Gene Symbols
APRFSTAT3
Kit Components
uncoated 96-well Microplate Wash Buffer A
Wash Buffer B
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho antibody
Anti-pan antibody
H
R
P-
Conjugated Secondary Antibody
T
M
B One-
Step Substrate
Stop Solution
Other Materials Required
Distilled or deionized water100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
Seed 10, 000-30, 000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at R
T with shaking.
Add 200 µl of prepared 1
X Quenching Buffer and incubate 20 min at R
T.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 °
C.
Add 50 µl of 1
X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at R
T.
Add 50 µl of prepared 1
X H
R
P-
Anti-
Rabbit or Mouse Ig
G and incubate for 1 h at R
T.
Add 100 µl of T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
Protein Name & Synonyms
Signal transducer and activator of transcription 3 (Acute-phase response factor)
Pathway
JAK/STAT Signaling

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5 Plate Kit
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 25.04.2024 

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