|
Hersteller |
Raybiotech
|
|
Kategorie |
|
|
Typ |
Elisa-Kit |
|
Specific against |
Human, Mouse, Rat |
|
Menge |
1 x 96-Well Microplate Kit |
|
ArtNr |
CBEL-STAT6-1 |
|
eClass 6.1 |
32160605 |
|
eClass 9.0 |
32160605 |
|
Lieferbar |
 |
| Quantity options |
|
| Short description |
RayBio Cell-Based Human/Mouse Stat 6 (Tyr641) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines. |
| Target Name and Synonyms |
STAT 6 |
| Solid Support |
96-well Microplate |
| Design Principle |
Cell-based |
| Result Output |
Semi-Quantitative |
| Method of Detection |
Colorimetric |
| Compatible Sample Types |
Adherent Cell Culture |
| Specificity |
The antibody pair provided in this kit recognizes human and mouse Stat 6 (pTyr641). |
| Product Features |
- Site and signal pathway-specific
- In vitro detection of adherent cell culture
- No sample lysis needed
- Compatible with a standard ELISA plate reader
- Faster results than with ELISA
- Adaptable for high-throughput screening and drug discovery
|
| Kit Components |
- Cell Culture Microplate
- Wash Buffer A
- Wash Buffer B
- Fixing Solution
- HRP-Conjugated Secondary Antibody
- Blocking Buffer
- TMB One-Step Substrate
- Stop Solution
- Quenching Buffer
|
| Other Materials Required |
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
|
| Protocol Summary |
- Seed 10, 000-30, 000 cells into each well and incubate overnight.
- Apply various treatment, inhibitors or activators according to manufacture's instructions.
- Add 100 ul of Fixing Solution into each well and incubate for 20 min at RT with shaking.
- Add 200 ul of prepared 1X Quenching Buffer and incubate 20 min at RT.
- Add 200 ul of Blocking Solution and incubate for 1 h at 37 C.
- Add 50 ul of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
- Add 50 ul of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
|
| Storage |
-20C |
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