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Human/Mouse/Rat Phospho-MEK1 (S217/S221) and Total MEK1 ELISA

Human/Mouse/Rat Phospho-MEK1 (S217/S221) and Total MEK1 ELISA

Hersteller	Raybiotech
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Human, Mouse, Rat
Menge	1 Plate Kit
Host	Hamster - Armenian
ArtNr	PEL-Mek-S217-T-1
Targets	MAP2K1
eClass 6.1	32160605
eClass 9.0	32160605
Lieferbar
Species Detected	Human Mouse Rat
Compatible Sample Types	Cell Lysates Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Gene Symbols	MAP2K1MEK1PRKMK1
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Anti- Phospho Antibody Anti- Pan Antibody H R P- Conjugated Secondary Antibody Streptavidin- Conjugated H R P Assay Diluent T M B One- Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Other Materials Required	Distilled or deionized water100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at R T or O/ N at 4 ° C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared 1 X H R P- Streptavidin to each well. Incubate 1 h at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (MKK1) (EC 2.7.12.2) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK 1)
Pathway	MAPK Signaling

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