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Human Phosphotyrosine FGFR1 ELISA Kit

Human Phosphotyrosine FGFR1 ELISA Kit

ArtNr	PEL-FGFR1-Y-1
Hersteller	Raybiotech
Menge	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-FGFR1.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	FGFR1
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Phosphorylation ELISA
Manufacturer - Targets	FGFR1 (P-Tyr)
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	RayBio® Human Phosphotyrosine FGFR1 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine FGFR1 in lysate samples.
Compatible Sample Types	Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Solid Support	96-well Microplate
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Biotinylated Anti-Phosphotyrosine Antibody Stop Solution Assay Diluent(s) Positive Control Sample Lysis Buffer Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 ul to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 ul of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 C. Add 100 ul of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
Result Output	Semi-Quantitative
UNSPSC Code	41116158

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

PEL-FGFR1-Y-1.pdf

PEL-FGFR1-Y-1-datasheet.pdf

PEL-FGFR1-Y-1-safety-datasheet.pdf