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Human Phospho-HDAC4 (S632) and Total HDAC4 ELISA Kit

Human Phospho-HDAC4 (S632) and Total HDAC4 ELISA Kit

ArtNr	PEL-HDAC4-S632-T-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Phosphorylation ELISA\|PTM ELISA
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	The RayBiotech Phospho-HDAC4 (S632) Kit measures phosphorylated HDAC4 (Ser632) Kit and total (pan) HDAC4 in cell and tissue lysates.
Compatible Sample Types	Cell Lysates Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Gene Symbols	HDAC4KIAA0288
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody Anti-Pan Antibody HRP-Conjugated Secondary Antibody Streptavidin-Conjugated HRP Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1 X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
UNSPSC Code	41116158
Protein Name & Synonyms	Histone deacetylase 4 (HD4) (EC 3.5.1.98)
Manufacturer - Specificity	The antibody pair provided in this kit recognizes human HDAC4 phosphorylated at site Serine-632 and total HDAC4.
Pathway	Epigenetics/chromatin remodeling
Introduction	RayBio® Phospho-HDAC4 (Ser632) and Total HDAC4 (Histone deacetylase 4) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated HDAC4 (Histone deacetylase 4) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-HDAC4 and total HDAC4. An anti-pan HDAC4 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HDAC4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-HDAC4 (Ser632) antibody is used to detect phosphorylated HDAC4 or mouse anti-HDAC4 antibody is used to detect pan HDAC4. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HDAC4 (Ser632) or pan HDAC4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Positive Control	Jurkat cells were treated with PV (Pervanadate). Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. Please see step 3 of Part VI Reagent Preparation for detail.
Stimulation	293 cells were treated with EGF. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

PEL-HDAC4-S632-T-5.pdf

PEL-HDAC4-S632-T-5-datasheet.pdf

PEL-HDAC4-S632-T-5-safety-datasheet.pdf