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Human Phosphotyrosine LCK ELISA Kit

Human Phosphotyrosine LCK ELISA Kit

ArtNr	PEL-LCK-Y-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology By Cell Type Hematology Lymphoma Myeloma ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	LCK
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Phosphorylation ELISA
Manufacturer - Targets	LCK
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	RayBio® Human Phosphotyrosine LCK ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated LCK in cell lysate samples.
Compatible Sample Types	Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Gene Symbols	LCK
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Biotinylated Anti-Phosphotyrosine Antibody Stop Solution Assay Diluent(s) Positive Control Sample Lysis Buffer Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1 X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
UNSPSC Code	41116158
Protein Name & Synonyms	Tyrosine-protein kinase Lck (EC 2.7.10.2) (Leukocyte C-terminal Src kinase) (LSK) (Lymphocyte cell-specific protein-tyrosine kinase) (Protein YT16) (Proto-oncogene Lck) (T cell-specific protein-tyrosine kinase) (p56-LCK)
Manufacturer - Specificity	This ELISA kit recognizes human LCK phosphotyrosine.
Pathway	T Cell ReceptorTyrosine Kinase Family

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

PEL-LCK-Y-2.pdf

PEL-LCK-Y-2-datasheet.pdf

PEL-LCK-Y-2-safety-datasheet.pdf