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Human ACE (CD143) ELISA Kit

Human ACE (CD143) ELISA Kit

ArtNr	ELH-ACE-5
Hersteller	Raybiotech
Menge	5 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Neuroscience Aging & Neurological Disorders Infectious Diseases Viral Infections Coronavirus (COVID-19) ELISA Cardiovascular Research Heart Failure Myocardial Infarction ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	0.084 ng/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Angiotensin-converting enzyme (ACE) (EC 3.2.1.-) (EC 3.4.15.1) (Dipeptidyl carboxypeptidase I) (Kininase II) (CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme, soluble form]
Versandbedingung	Gekühlt
Lieferbar
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin1 (CT-1), Cathepsin V, FABP1 (L-FABP), FGF-20, GDF-8 (Myostatin), HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kalli
Manufacturer - Category	COVID-19\|ELISA Kits\|ELISA Kits\|Sandwich ELISA
Shipping Temperature	Blue ice
Storage Conditions	?-20°C
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	ACEDCPDCP1
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try Ray Biotech's Speed ELISA platform for quantitative detection in just three hours.
Protein Name & Synonyms	Angiotensin-converting enzyme (ACE) (EC 3.2.1.-) (EC 3.4.15.1) (Dipeptidyl carboxypeptidase I) (Kininase II) (CD antigen CD143) [Cleaved into: Angiotensin-converting enzyme, soluble form]
UNSPSC Code	41116158
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin1 (CT-1), Cathepsin V, FABP1 (L-FABP), FGF-20, GDF-8 (Myostatin), HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1 (Tripeptidyl-peptidase I), TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP.
Recommended Dilution	100 fold
Additional Notes	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELH-ACE-5.pdf

ELH-ACE-5-datasheet.pdf

ELH-ACE-5-safety-datasheet.pdf