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Mouse VEGF ELISA Kit

ArtNr ELM-VEGF-2
Hersteller Raybiotech
Menge 2 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Mouse (Murine, Mus musculus)
Host Hamster - Armenian
Sensitivity 2 pg/ml
Citations 1. Saldaña L, Crespo L, Bensiamar F, et al. Mechanical forces regulate stem cell response to surface topography. J Biomed Mater Res A. 2013 Apr 24, [Epub ahead of print] doi:10.1002/jbm.a.34674.
2. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1, 123(2): 751–766.
3. Xiao X, Guo P, Chen Z, et al. Hypoglycemia reduces vascular endothelial growth factor A production by pancreatic beta cells as a regulator of beta cell mass. J Biol Chem. 2013 Mar 22,288(12):8636–8646.
4. Martin D, Nguyen Q, Molinolo A, Gutkind JS. Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene. Oncogene. 2013 May 27, [Epub ahead of print] doi: 10.1038/onc.2013.193.
5. Hartwich J, Orr WS, Ng CY, et al. HIF-1alpha activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan,48(1):39–46.
6. Hartwich J, Orr WS, Ng CY, et al. HIF-1alpha activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan,48(1):39–46.
7. Bhuvaneswari R, Gan Y, Yee K, Soo K,Olivo M. Effect of hypericin-mediated photodynamic therapy on the expression of vascular endothelial growth factor in human nasopharyngeal carcinoma. Int J Mol Med. 2007,20:421-428.
8. Nasr M., Nafee N., Saad H., Kazem A. Improved antitumor activity and reduced cardiotoxicity of epirubicin using hepatocyte-targeted nanoparticles combined with tocotrienols against hepatocellular carcinoma in mice. European Journal of Pharmaceutics and Biopharmaceutics. Available online 6 May 2014, http://dx.doi.org.proxy.library.emory.edu/10.1016/j.ejpb.2014.04.016 [Epub ahead of print]
9. Hartwich J., Orr S., Ng C., Spence Y., Morton C., DavidoffA. HIF-1[alpha] activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. Journal of Pediatric Surgery Volume 48, Issue 1 , Pages 39-46, January 2013
10. Konac E., et al. Does Pattern Scan Laser (PASCAL) photocoagulation really induce less VEGF expression in murine retina than conventional laser treatment? Gene. In Press, Corrected Proof, Available online 24 July 2014. http://dx.doi.org/10.1016/j.gene.2014.07.062
11. Abbas OL., et al. Inhibition of the Notch Pathway Promotes Flap Survival by Inducing Functional Neoangiogenesis. Annals of Plastic Surgery August 28, 2014, epub ahead of print. doi: 10.1097/SAP.0000000000000197
12. Wei, Z., Yu, S. et al. Regulatory Role of the JNK-STAT1/3 Signaling in Neuronal Differentiation of Cultured Mouse Embryonic Stem Cells. Cellular and Molecular Neurobiology 2014. Epub Ahead of Print: DOI: 10.1007/s10571-014-0067-4
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Similar products VEGF-A
Versandbedingung Gekühlt
Lieferbar
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 bet
Manufacturer - Category
ELISA Kits|Sandwich ELISA
Manufacturer - Targets
VEGF-A
Shipping Temperature
Blue ice
Storage Conditions
?-20°C
Description
RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Design Principle
Sandwich-based
Method of Detection
Colorimetric
Solid Support
96-well Microplate
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 ul to 1 ul volumes
Adjustable 1-25 ul pipettes for reagent preparation
100 ul and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 ul of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4C.
Add 100 ul of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 ul of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 ul of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 ul of Stop Solution to each well.
Read at 450 nm immediately.
Product Features
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Result Output
Quantitative
UNSPSC Code
41116158
Manufacturer - Specificity
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Recommended Dilution
3 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 Plate Kit
Lieferbar: In stock
lieferbar

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