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Human Phospho-TAK1 (Ser412) and Total TAK1 Cell-Based ELISA Kit

ArtNr CBEL-TAK1-S412-1
Hersteller Raybiotech
Menge 1 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens)
Host Hamster - Armenian
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Specificity This ELISA kit recognizes Human TAK1 phosphorylated at site Serine-412 as well as total TAK1.
Manufacturer - Category
Assay Kits|Phosphorylation Assays|ELISA Kits|Cell-Based ELISA
Shipping Temperature
Blue ice
Storage Conditions
-20°C
Compatible Sample Types
Adherent Cell Culture
Design Principle
Cell-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Semi-Quantitative
Solid Support
96-well Microplate
Gene Symbols
MAP3K7TAK1
Kit Components
Uncoated 96-well Strip Microplate
Wash Buffers
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution
Other Materials Required
Distilled or deionized water 100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
Seed 10, 000-30, 000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1
X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
Add 50 µl of 1
X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1
X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Product Features
Rapidly measure phosphorylated protein in adherent cell lines
Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader
Protein Name & Synonyms
Mitogen-activated protein kinase kinase kinase 7 (EC 2.7.11.25) (Transforming growth factor-beta-activated kinase 1) (TGF-beta-activated kinase 1)
UNSPSC Code
41116158
Manufacturer - Specificity
This ELISA kit recognizes Human TAK1 phosphorylated at site Serine-412 as well as total TAK1.
Pathway
MAPK SignalingNFkB Signalingp53 SignalingTGF-beta SignalingWnt/beta-Catenin Signaling
Calyculin A and IL-1 beta Treatment of Hela Cell Line
HeLa cells were treated with Calyculin A and IL-1 beta

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 Plate Kit
Lieferbar: In stock
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