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Neurotensin ELISA Kit

ArtNr EIA-NT-2
Hersteller Raybiotech
Menge 2 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Hamster - Armenian
Sensitivity 0.2 ng/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Category
ELISA Kits|Competitive ELISA
Manufacturer - Targets
Neurotensin
Shipping Temperature
Blue ice
Storage Conditions
-20°C
Description
RayBio® Human/Mouse/Rat Neurotensin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
Compatible Sample Types
Plasma, Serum
Design Principle
Competition-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
NTS
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
HRP-Streptavidin
TMB One-Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Sigma
Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
UNSPSC Code
41116158
Protein Name & Synonyms
Neurotensin/neuromedin N [Cleaved into: Large neuromedin N (NmN-125) Neuromedin N (NN) (NmN) Neurotensin (NT) Tail peptide]
Manufacturer - Specificity
This EIA kit is designed to detect human, mouse, and rat Neurotensin .
Recommended Dilution
Human: 2X / Mouse: 8X / Rat: 8X
Introduction
Neurotensin is a 13 amino acid neuropeptide that is widely distributed throughoutthe nervous system, and may function as a neurotransmitter or a neuromodulator. Itacts on three subtypes of neurotensin receptors which have many central andperipheral actions.Neurotensin induces a variety of effects, including: analgesia, hypothermia andincreased locomotor activity. It may be involved in dopamine-associatedpathophysiological events.In the periphery, Neurotensin is found in enteroendocrine cells of the smallintestine. It may be involved in the maintenance of gut structure and function, and inthe regulation of fat metabolism. It is a potent mitogen for colorectal cancer.Neurotensin also exhibits antimicrobial activity against bacteria and fungi.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 Plate Kit
Lieferbar: In stock
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