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Human Caspr2 Immuno-PCR Assay Kit, IQELISA®

Human Caspr2 Immuno-PCR Assay Kit, IQELISA®

ArtNr	IQH-CASPR2-1
Hersteller	Raybiotech
Menge	1 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Neuroscience Neural Lineage Markers ELISA ELISA & Immunoassays ELISA Kits Immunoassay Neuronal Cell Markers
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
Sensitivity	Typically 10x lower than traditional ELISA
Dry ice	Yes
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Versandbedingung	Trockeneis
Lieferbar
Manufacturer - Category	ELISA Kits\|Immuno-PCR Assays
Shipping Temperature	Dry ice (additional shipping fee)
Storage Conditions	-20°C
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	qPCR
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate **The PCR plate used is a 0.2mL, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600)
Gene Symbols	CASPR2CNTNAP2KIAA0868
Kit Components	Caspr2 Microplate (Item A): 96 well PCR plate coated with anti-Human Caspr2 Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution Standards (Item C): 2 vials of recombinant Human Caspr2 Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent Detection Affinity Reagent for Caspr2 (Item F): 2 vials of a 4x concentrated solution of anti-Human Caspr2 affinity reagent IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock Primer Solution (Item I): 1.7 ml vial PCR Master Mix (Item J): 1.2 ml vial PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer The PCR plate used is a 0.2mL, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600)
Other Materials Required	Real-time PCR instrument, Bio-Rad recommended Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders Absorbent paper Distilled or deionized water Log-log graph paper or computer and software for data analysis Tubes to prepare standard or sample dilutions Heating block or water bath capable of 80°C
Protocol Outline	Prepare all reagents, samples and standards as instructed Add 25µl standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C Add 25 µl detection affinity reagent to each well. Incubate 1 hour at room temperature Add 25µL of IQELISA Detection Reagent to each well. Incubate 1 hour Add 15µL Primer solution 10µL of PCR master mix to each well Run real-time PCR
Protein Name & Synonyms	Contactin-associated protein-like 2 (Cell recognition molecule Caspr2)
UNSPSC Code	41116158
Introduction	The RayBio® Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio® Custom Human Caspr2 IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Caspr2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Caspr2 coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and Caspr2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.

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