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Human Phospho-Cyclin B1 (S126) ELISA Kit

Human Phospho-Cyclin B1 (S126) ELISA Kit

ArtNr	PEL-CYCLINB1-S126-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Oncology Neuroscience Neural Lineage Markers By Cell Type Blastoma Carcinoma Cell Biology Cell Status ELISA Proliferation ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer
Typ	Elisa-Kit
Specific against	Human (Homo sapiens)
Host	Hamster - Armenian
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	Assay Kits\|Phosphorylation Assays\|ELISA Kits\|Phosphorylation ELISA\|PTM ELISA
Manufacturer - Targets	Cyclin B1
Shipping Temperature	Blue ice
Storage Conditions	-20°C
Description	The RayBiotech Human Phospho-Cyclin B1 (S126) ELISA Kit measures phosphorylated Cyclin B1 (Ser126) and total (pan) Cyclin B1 in cell and tissue lysates.
Compatible Sample Types	Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Gene Symbols	CCNB1
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Other Materials Required	Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 µl to 1 ml volumes Adjustable 1-25 ml pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline	Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1 X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
UNSPSC Code	41116158
Protein Name & Synonyms	G2/mitotic-specific cyclin-B1
Manufacturer - Specificity	The antibody pair provided in this kit recognizes Human Cyclin B1 phosphorylated at site Serine-126.
Pathway	AMPK SignalingCell CycleDNA Damage
Positive Control	HeLa cells were treated with Nocodazole at 37°C for 20 hours. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA (see Reagent Preparation step 4).
Stimulation	HeLa cells were untreated or treated with Nocodazole for 20 hours. Cell lysates were analyzed using this phosphoELISA and Western Blot.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

PEL-CYCLINB1-S126-2.pdf

PEL-CYCLINB1-S126-2-datasheet.pdf

PEL-CYCLINB1-S126-2-safety-datasheet.pdf