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Rat IL-1 beta ELISA Kit

Rat IL-1 beta ELISA Kit

ArtNr	ELR-IL1b-2
Hersteller	Raybiotech
Menge	2 Plate Kit
Quantity options	1 Plate Kit 2 Plate Kit 5 Plate Kit 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie	Neuroscience Neuroinflammation Metabolism Cholesterol & Lipid Metabolism Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Host	Hamster - Armenian
Sensitivity	80 pg/ml
Citations	1. Seif A., Abdelwahed D. Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats. Journal of Physiology and Biochemistry, April 22, 2014 doi.10.1007/s13105-014-0335-2 [Epub Ahead of Print] 2. Kumar B., et al. Retinal neuroprotective effects of quercetin in streptozotocin-induced diabetic rats. Experimental Eye Research 2014 Epub Ahead of Print on 18 June DOI:10.1016/j.exer.2014.06.009 3. Mu X., et al. Protective Effects of Carbon Monoxide-Releasing Molecule-2 on the Barrier Function of Intestinal Epithelial Cells . PLOS One Published: August 07, 2014 DOI: 10.1371/journal.pone.0104032 4. Kumar A., et al. Effect of Licofelone—A Dual COX/5-LOX Inhibitor in Intracerebroventricular Streptozotocin-Induced Behavioral and Biochemical Abnormalities in Rats. Journal of Molecular Neuroscience. Sept 2014, epub ahead of print. DOI 10.1007/s12031-014-0414-4 5. Srivastava M., et al. Nanoemulgel (NEG) of Ketoprofen with eugenol as oil phase for the treatment of ligature-induced experimental periodontitis in Wistar rats. Drug Delivery Sept 2014, epub ahead of print.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Similar products	IL-1 beta, IL-1 F2
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Category	ELISA Kits\|Sandwich ELISA
Manufacturer - Targets	IL-1 beta / IL-1F2
Shipping Temperature	Blue ice
Storage Conditions	≤-20°C
Description	RayBio® Rat IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Lead time: Typically ships within 1-2 business days. No Friday shipments.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Gene Symbols	IL1B
Kit Components	Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 ul of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4C. Add 100 ul of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 ul of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 ul of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 ul of Stop Solution to each well. Read at 450 nm immediately.
Product Features	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
UNSPSC Code	41116158
Result Output	Quantitative
Protein Name & Synonyms	Interleukin-1 beta (IL-1 beta)
Manufacturer - Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Recommended Dilution	2 fold

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

ELR-IL1b-2.pdf

ELR-IL1b-2-datasheet.pdf

ELR-IL1b-2-safety-datasheet.pdf